总PSA时间分辨免疫荧光分析法的建立  

Time-resolved fluoroimmunoassay for the total detection of prostate-specific antigen

作　　者：杭建峰[1] 吴英松[1] 徐伟文[1] 李明[1] 

机构地区：[1]南方医科大学热带医学研究所,广州510515

出　　处：《现代免疫学》2005年第5期402-405,共4页Current Immunology

基　　金：广州市研究开发基地建设资助项目(2003U130021)

摘　　要：利用时间分辨免疫荧光分析(TRFIA)技术建立人血清总PSA(前列腺特异性抗原)的快速全自动检测方法及其诊断试剂研制,该法采用双抗体夹心法建立tPSA-TRFIA,对该法和研制试剂的指标评价表明:方法的线性测量范围为0.50～250μg/L,灵敏度为0.06μg/L,批内批间的精密度分别为5.58%～9.71%,6.49%～12.12%。与CEA、CA12-5、CA15-3、CA19-9、AFP无交叉反应。353份血清标本用本试剂与国外其他试剂同时检测,其相关性达到94.4%,结果表明,试剂测定各项指标均达到临床检测要求,可替代国外同类产品。A rapid and full-automatized method by using the time-resolved fluoroimmunoassay （TRFIA） to detect the total prostate-specific antigen （tPSA） in human sera was established and its diagnostic reagents were prepared in the present study. The double antibody sandwich assay was employed in this method of detecting the presence of tPSA in human sera. From the evaluation of the reagent indices, it appeared that the linear test range of this method was 0.50-250 μg/L, and its sensitivity of testing was 0.06μg/L. The intra- and inter-batch accuracies were 5.58%-9.71% and 6.49%-12.12% respectively. No cross-reaction could be demonstrated with CEA, CA12- 5, CA15-3, CA19-9 and AFP. The results of a simultaneous testing with other foreign reagents of the same character indicated that the correlation among them was 94.4%. All these indices approach to the requirement for the clinical use of testing.

关 键 词：总PSA(前列腺特异性抗原) 时间分辨免疫荧光分析(TRFIA) 

分 类 号：R392[医药卫生—免疫学]