东北春玉米骨干自交系组织培养再生系统研究  

Study on Regeneration of Spring Maize Inbred Lines in Northeast of China

作　　者：袁鹰[1] 曲喜云 王玉民[1] 郝文媛[1] 谭化[1] 张光弟[3] 刘德璞[1] 

机构地区：[1]吉林省农业科学院生物技术重点实验室,吉林公主岭136100 [2]长春市种子管理站 [3]吉林省梨树县农业技术推广站

出　　处：《玉米科学》2005年第3期28-31,共4页Journal of Maize Sciences

基　　金：国家转基因植物中试及产业化基地(吉林)专项资助(JY03-17-1)

摘　　要：东北玉米骨干自交系在诱导愈伤组织和植株再生中,基因型差异非常显著。自交系7922最高愈伤诱导率达89.1%,芽苗率为66.4%;而最低的921诱导率仅有18.9%,芽苗率为0。再生途径主要有两种:7922主要以体胚发生途径为主,4122、8902主要以器官发生途径为主。2熏4-D是体胚诱导不可缺少的植物生长激素,适当的细胞分裂素能促进诱导4112、8902不定芽的产生。而且,再生途径并不是一成不变的,在一定条件下可以相互转变。There existed great differences among maize genotypes when immature embryos were cultured for plant regeneration. 7922 showed the highest frequencies of callus induction and shoot formation which were 89.1%, and 66.4% respectively. While the lowest was found in 921, the induction and regeneration frequencies were 18.9% and 0 respectively. There are two regeneration pathways. 7922 tended to regenerate via somatic embryogenesis, 4112, 8902 are likely to regenerate via organogenesis. 2,4-D was essential for inducing somatic embryoids, and suitable cytokin can promote adventitious shoot formation of 4112 and 8902. Furthermore, the regeneration pathways are interchangeable under different culture conditions.

关 键 词：玉米 自交系 组织培养 再生系统 

分 类 号：S513.035[农业科学—作物学]