三丁基锡对正常人胚胎羊膜细胞凋亡的诱导作用  

Study of Tributyltin-induced apoptosis in human amniotic cells

作　　者：朱欣[1] 应李强[2] 徐进[1] 徐立红[1] 

机构地区：[1]浙江大学劳动卫生与环境卫生研究所,杭州310031 [2]浙江大学医学院生物化学与分子生物学教研室,杭州310031

出　　处：《环境科学学报》2005年第9期1250-1252,共3页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.20137010)~~

摘　　要：为了探讨三丁基锡(TBT,tributyltin)对正常人羊膜细胞FL(humanamnioticcells)凋亡的诱导作用,进行了流式细胞仪PIAnnexinⅤ双染色检测TBT对FL细胞凋亡的诱导作用,并采用荧光染料FITC标记的特异性的caspase3的抑制剂检测活细胞中caspase3的酶活性.实验结果表明,0、1、2、3、4μmol·L-1浓度的三丁基锡作用于FL细胞2h后,细胞凋亡率和细胞中caspase3酶活性都明显升高,且有良好的剂量效应关系.表明三丁基锡在一定浓度和暴露时间下能够诱导FL细胞的凋亡,而且在此过程中,caspase3起重要作用.The objective of present study was to investigate the effect of tributyhin on apeptosis in FL（ human anmiotic cells）cells. The PI/AnnexinV flow cytometry method was used to study the apeptosis induction of tributyhin on FL cells and the enzyme activity of caspase-3 in live cells was detected by fluorescent dye labeled specific inhibitor of caspase-3. The results showed that after exposed to 0,1,2,3,4 μmol/L TBT for 2h, both the apeptotic rate and caspase-3 activity in FL cells were increased in a dose-dependent manner. The results proved that tributyhin could induce apeptosis of FL cells, and caspase-3 played an central role in this process as well.

关 键 词：三丁基锡 细胞凋亡 FL细胞 PI/Annexin V双染色 CASPASE-3 

分 类 号：R994.6[医药卫生—毒理学]