葡萄糖转移方法检测脂肪颗粒活性的研究  

Study on viability measurement of fat for grafting

作　　者：雷华[1] 李青峰[1] 

机构地区：[1]上海第二医科大学附属第九人民医院整形外科,200011

出　　处：《中华整形外科杂志》2005年第5期375-378,共4页Chinese Journal of Plastic Surgery

基　　金：上海市科技发展基金资助项目(034119826)

摘　　要：目的以高速离心为损伤模型,了解以葡萄糖转移量来体现组织块活性的可能性、可靠性,建立新的脂肪颗粒活性检测方法,为脂肪移植研究提供一基础。方法将同条件抽取的脂肪颗粒分为5组,分别经1000、2000、3000、4000、5000rmin离心纯化后,每组得60ml样本,每个样本分为12个实验标本(5ml标本)置于12个培养皿中(内含10mlDMEM、1U正规胰岛素皿),每组设一个不含脂肪颗粒的空白标本,所有标本同时进行孵育,1h后测量每个标本DMEM中葡萄糖的浓度。以5组空白标本的葡萄糖浓度均值为基础浓度,其与每个实验标本的葡萄糖浓度差值代表标本的葡萄糖转移量即活性。t检验分析比较每组标本的活性。将5组样本送病理学检查,比较各组切片完整细胞数,并与上述实验结果进行对比。结果各组样本经含糖DMEM孵育后,其葡萄糖转移量即活性用SNK方差分析检验得出:1000rmin组>2000rmin组>3000rmin组>4000rmin组,5000rmin组与4000rmin组比较,差异无统计学意义,但显著小于前4组(P<0.05),即随离心速率增大,脂肪颗粒活性逐渐下降。病理切片显示:5000rmin组大部分细胞壁形态不规则或碎裂。完整细胞计数结果用SNK方差分析检验得出:5000rmin组明显较其他各组少(P<0.05);其他4组完整细胞数差异无统计学意义(P>0.05)。结论葡萄糖转移实验可较准确地检测脂肪颗粒活性的活性状态,病理学检查在反应脂肪颗粒活性方面有局限性。Objective To establish a new method to accurately measure the viability of fat for grafting, whieh can lay a foundation for the further study, of fat transplantation. Methods We ceutrifugated 5 groups of fat respectively at 1 000 r/min, 2 000 r/min, 3 000 r/min, 4 000 r/min and 5 000 r/min, took out 12 samples from per grnup of eeatrifugated fat, 5 ml per sample, and put them into dishes to incubate with DMEM containing glucose for nne hour. Simultaneously we set up 5 control dishes （only containing DMEM without fat） to incubate together with those experimental dishes. After incubating we measured the glueose eonsistenee of DMEM in all dishes. The glucnse consistetence difference between the mean value of 5 control dishes and per experimental dish represented the glucose transportation quantity of per sample, in other words, the sample viability. We analyzed and compared the glucose transportation qaantity of every group of samples with t test. The 5 groups of fat were also put in histopathnlogie examiuation and the amounts of intact adipoeytes on per group of slides were compared with T test. Results The glucose transportation quantities of group 1 000 r/min,2 000 r/min, 3 000 r/min, 4 000 r/min, 5 000 r/min gradually deereased in turn （P 〈 0.05）. The histopathologie examination didn＇t show the difference among group 1 000 r/min, 2000 r/min. 3 000 r/min and 4 000 r/min the 5 000 r/min （ P 〉 0.05）, and only showed the amounts of intact adipocytes on slides of group 5 000 r/min were significantly fewer than other four group （ P 〈 0.05）. Conclusion The glucose transportation test can he used to accurately measure the viability of fat. The histopathnlogic examination can reveal fat viability roundly.

关 键 词：脂肪移植 脂肪颗粒活性 葡萄糖转移实验 葡萄糖浓度 活性状态 脂肪颗粒 检测方法 转移 实验标本 病理学检查 高速离心 DMEM 

分 类 号：R622[医药卫生—整形外科]