水飞蓟宾-卵磷脂复合物对脑缺血再灌注损伤保护作用的研究  

Influence of Expression of Intercellular Adhesion Molecule-1 on Cerebral Ischemic Reperfusion Injury and Cerebral Protective of Silybin-lecithin Compound in Mongolian Gerbil

作　　者：李玮[1] 吴晓华[1] 陆红梅[1] 管阳太[2] 

机构地区：[1]上海市第七人民医院神经科 [2]中国人民解放军第二军医大学附属长海医院神经科

出　　处：《齐齐哈尔医学院学报》2005年第9期998-1000,共3页Journal of Qiqihar Medical University

摘　　要：目的探讨细胞间粘附分子(ICAM-1)表达与中性粒细胞(PMNs)浸润、缺血再灌注损伤的关系,并观察水飞蓟宾-卵磷脂复合物(silybin-lecithin compound,SLC)对缺血再灌注损伤的保护作用机理.方法蒙古沙土鼠155只.分设:1)缺血组;2)缺血再灌注组;3)SLC100mg/kg组;4)SLC200mg/kg组;5)SLC400mg/kg组;6)假手术对照组;7)溶剂组.除假手术对照组外,每组分设1、3、6、12、24h时相点.取鼠脑组织块用原位杂交和免疫组化检测方法测定ICAM-1mRNA及其蛋白表达水平,用酶法测定PMNs浸润数.结果脑缺血再灌注时,ICAM-1表达明显增高,其中ICAM-1mRNA表达至6h达高峰,而ICAM-1蛋白质表达水平、PMNs浸润数改变于12～24h达最高峰,二者之间呈显著正相关,但ICAM-1mRNA与后二者间无明显相关性.SLC组上述指标于再灌注时显著下降,其下降程度与时相和浓度呈明显的负相关.均P<0.01或0.05.结论脑缺血再灌注时,ICAM-1参与介导了PMNs对组织细胞的粘附、浸润和再灌注损伤的发生发展,SLC可通过抑制ICAM-1的表达而产生神经细胞的保护作用.Objective To investigate the influence of expression of intercellular adhesion molecule -1 （ICAM-1） on cerebral isehemie reperfusion injury and cerebral infiltration of polymorphonuclear neutrophils （PMNs）. Methods using an established common carotid artery occlusion model.we observed the expression of ICAM- 1 by in setup hybridization and the protein by immunocytochemistry, and the numbers of infiltrated PMNs were measured with MPO enzyme marker method. Results After cerebral isehemie reperfusion,eerebral levels of expression of ICAM- 1 were increased significantly and reached a peak at 6h;but the ICAM- 1 protein peaked during 12-24h. Number of PMNs also increased and peaked after reperfusion 12-24h. Close positive correlation （P 〈 0.05） was found between the levels of expression of ICAM- 1 and PMNs. In SLC group,all of the indices were increased after isehemie reperfusion,but the levels of increase were significantly lower （P 〈 0. 05 or P 〈 0.01） as compared with PVP group. Conclusions The findings indicate that ICAM-1 and PMNs is implicated in the development of cerebral isehemie reperfusion injuries. SLC could protect the cerebral tissue from cerebral isehemie reperfusion by partially blocking the expression of ICAM- 1.

关 键 词：水飞蓟宾-卵磷脂复合物 缺血再灌注 细胞间粘附分子-1 原位杂交 蒙古沙土鼠 水飞蓟宾-卵磷脂复合物 脑缺血再灌注损伤 保护作用 ICAM-1MRNA 细胞间粘附分子 ICAM-1表达 蛋白表达水平 免疫组化检测 PMNS 

分 类 号：R975.5[医药卫生—药品] R743.3[医药卫生—药学]