高糖及糖尿病患者白细胞对内皮细胞明胶酶的影响  

A preliminary study on the effects of high glucose and the leucocyte of diabetic patients on endothelial cells

作　　者：郭立新[1] 孙明晓[1] 李东晓[1] 周迎生[1] 王晓霞[1] 李慧[1] 

机构地区：[1]卫生部北京医院内分泌科,100730

出　　处：《中华老年医学杂志》2005年第9期652-654,共3页Chinese Journal of Geriatrics

摘　　要：目的观察高糖环境及糖尿病患者白细胞与内皮细胞共同孵育后白细胞与内皮细胞的黏附率及92000明胶酶(MMP-9)的变化规律。方法培养人脐静脉内皮细胞,用不同浓度的葡萄糖培养液共同孵育,计算细胞黏附率和应用ELISA方法测定MMP-9水平。结果高糖环境可使白细胞与内皮细胞黏附率增加,随共育时间的延长此作用加强。在高糖环境中培养的内皮细胞MMP-9水平显著升高,加入糖尿病患者白细胞后此种趋势更加明显。结论高糖环境促使内皮细胞分泌MMP-9增加,糖尿病患者白细胞对内皮细胞具有细胞毒作用。Objective To study the effects of high glucose and the leucocyte of diabetic patients on endothelial cells. Methods The leucocyte adhesion percentage to endothelial cells and the MMP-9 expression of co-culturing endothelial cells and leucocytes were determined under different levels of glucose and at different time. Results （1） The level of MMP-9 was much higher under high glucose culture, and it increased as culture time prolonged. （2） The percentage of leucocyte adhesion to endothelial cells under high level of glucose was increased, and it was further enhanced when co-culture with leucocytes of diabetic patients . （3） The level of MMP-9 in supernatant of endothelial cells and leucocytes was increased significantly after culture with leucocytes of diabetic patients. It showed leucocytes were toxic to endothelial cells. Conclusions High glucose increases the level of MMP-9 of endothelial cells and percentage of the leucocyte adhesion to endothelial cells. Leucocytes of diabetic patients are toxic for endothelial cells.

关 键 词：明胶酶B 内皮细胞 葡萄糖溶液 高渗 内皮细胞明胶酶 糖尿病患者 高糖环境 白细胞 人脐静脉内皮细胞 MMP-9 ELISA方法 

分 类 号：R587.1[医药卫生—内分泌]