枯草芽孢杆菌B-903菌株的诱变选育  

Mutation Breeding of Bacillus subtilis B-903 Producing High-yield Substance

作　　者：王瑞霞[1] 刘红彦[1] 刘玉霞[1] 王泽云[2] 李洪连[3] 

机构地区：[1]河南省农业科学院植物保护研究所,郑州450002 [2]河南科技学院,新乡453003 [3]河南农业大学,郑州450002

出　　处：《微生物学通报》2005年第5期10-13,共4页Microbiology China

基　　金：国家科技攻关计划(No.2001BA701A58-01);河南省属科研院所研究专项资金项目(No.1050111)

摘　　要：枯草芽孢杆菌B-903菌株是由河南省农业科学院植物保护研究所从郑州果园中分离得到,其代谢产生的抗菌物质对多种植物病原真菌具有较强抑制作用。以此菌株为出发菌株,进行亚硝基胍(NTG)和微波诱变处理,确定了二者诱变处理的最佳处理剂量:NTG最佳处理浓度为200μg/mL,微波诱变为HI微波挡(850W、脉冲频率2450MHz)处理100s,筛选出13个高效突变株。经传代实验,2株高效突变株N1和W2抑菌圈直径分别稳定在26mm和24mm以上,比出发菌株提高21·8%和14·8%。Bacillus subtilis B-903 strain was selected from orchard in Zhengzhou by Institute of plant protection, Henan academy of agricultural sciences. It produces antagonistic substance which is strong antagonistic to various plant pathol pathogenic fungi. Using strain B-903 as starting strain, thirteen high-yield strains were obtained after 200 μg/mL NTG treatment and 100s （850W 2450MHz） Microwave irradiation. Through fermentation experiment in shaking flasks, inhibitory effect of N1 and W2 were above 26mm and 24ram steadily , increased respectively by 21.8% and 14. 8% compared with the starting strain.

关 键 词：枯草芽孢杆菌 诱变 亚硝基胍 微波 

分 类 号：Q933[生物学—微生物学]