高硝化活性亚硝酸盐氧化细菌的培养和应用研究  

Studies on the Culture and Applicayion of a Nitrite-oxidizing Bacterium with High Nitrification Activity

作　　者：琚姝[1] 周长林[1] 窦洁[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009

出　　处：《微生物学通报》2005年第5期56-61,共6页Microbiology China

摘　　要：针对本实验室筛选得到的一株亚硝酸盐氧化细菌（nitrite—oxidizing bacteria），研究了在28℃～30℃、摇床转速为110r／min时．pH、氮源、碳源、NaCl、有机物对菌体生长的影响。结果表明，培养基pH8．0～8．5、NaNO2含量4，500mg／L、Na2CO3含量1．5g／L、NaCl含量0～0．5％、葡萄糖含量0～0．1％时，亚硝酸盐氧化细菌生长良好，培养9d时，细菌浓度可达4．6×10^9MPN／mL，且培养基中的NO2^--N能全部被硝化为NO3^--N。培养基中NaCl含量大于0．5％、葡萄糖含量大于0．1％时，亚硝酸盐氧化细菌对氮源的利用受到抑制。亚硝酸盐氧化细菌降解淡水养殖池塘中的NO2^--N试验表明，在水温25℃、pH8．6的池塘中，NO2^--N从菌体投放后的第3d开始下降，18d后NO2^--N由1．47mol／L下降至0．49mol／L。As to a nitrite-oxidizing bacterium screened in our laboratory, the effect of pH, nitrogen sources, carbon sources and sodium chloride on its growth was studied in order to obtain high cell density. It showed that the culture conditions of nitrite- oxidizing bacterium were 4500mg/L sodium nitrite, 1.5g/L sodium carbonate, 0 ～0.5% sodium chloride and 0 ～0. 1% glucose at 28℃ ～30℃, 110 r/rain and pH 8.0 ～ 8.5. After cultivation for 9 days, the bacteria concentration reached 4.6 × 10^9 MPN/mL and all NO2^ -N in the medium was converted to NO3^-N. But the transformation of nitrogen sources was inhibited while the sodium chloride concentration exceeded 0.5% or glucose concentration exceeded 0. 1%. According to the test of NO2^- -N conversion with nitrite- oxidizing bacteria in a freshwater aquaculture pond, NO2^- -N began to decrease on the third day of the moculation of this bacterium and the concentration dropped from 1.47mol/L to 0.49mol/L after 18 days at 25℃ and pH 8.6.

关 键 词：亚硝酸盐氧化细菌 培养 亚硝酸盐转化 

分 类 号：Q939.99[生物学—微生物学]