梭子蟹乳化病的间接ELISA诊断  

Study on indirect ELISA method for detecting the pathogenic bacteria of Emulsification disease in portunidae

作　　者：赵青松[1] 徐镇[1] 陈寅儿[1] 金珊[1] 徐国辉 

机构地区：[1]宁波大学生物科学与生物工程学院 [2]浙江省舟山市普陀区海洋与渔业局,浙江舟山316010

出　　处：《上海水产大学学报》2005年第3期248-252,共5页Journal of Shanghai Fisheries University

基　　金：浙江省自然科学基金项目(302440);宁波市青年基金项目(2003A62013)

摘　　要：以梭子蟹乳化病的病原菌-溶藻弧菌为抗原,制成免疫血清;利用辣根过氧化酶标记的羊抗兔血清(HRP-IgG)为酶标二抗、建立了检测溶藻弧菌的间接ELISA快速诊断法。结果表明,应用间接ELISA技术检测溶藻弧菌有较高的灵敏度,最低的检测量为104cfu/mL。同时交叉反应表明其具有较高的特异性。人工感染试验48 h后,就能在血淋巴和鳃中检测到溶藻弧菌。应用建立的间接ELISA法进行现场检测,表观有病蟹的阳性检出率为90%,表观健康蟹的阳性检出率为20%,养殖海水中没有阳性检出。Presented in this paper is a method of indirect of enzyme linked immunosorbent assay （ELISA） for rapid diagnosis of pathogenic bacteria of Emulsification disease in portunidae. The antiserum for Vibrio alginolyticus was obtained by immunizing rabbit against Vibrio alginolyticus, the anti-rabbit IgG goat was marked horseradish peroxidase（HRP） and used in an indirect ELISA detection. The result showed that the lowest Vibrio alginolyticus suspension detected limit in laboratory was 10^4 CFU/mL. Vibrio alginolyticus was dectecting in 48h, artificial infection. On worksite, Vibrio alginolyticus was detected by indirect ELISA from the clinically diseased and healthy portunidae at the rate of 90% and 20% respectively, and none from the seawater.

关 键 词：梭子蟹 乳化病 间接EHSA 溶藻弧菌 

分 类 号：S945[农业科学—水产养殖]