几种瓜类枯萎病菌专化型的AFLP分析  

Analysis on the formae speciales of Fusarium oxysporum causing wilts of several cucurbits by AFLP

作　　者：段会军[1] 姬惜珠[1] 张彩英[1] 李兴红[2] 郭小敏[1] 马峙英[1] 

机构地区：[1]河北农业大学,河北保定071001 [2]北京市农林科学院,北京100089

出　　处：《河北农业大学学报》2005年第5期71-74,共4页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金资助项目(303188);河北省教育厅科研基金资助项目(2002141)

摘　　要：利用AFLP分子标记技术对西瓜、冬瓜、黄瓜和甜瓜4个不同专化型的10个菌株进行了分析,结果表明10对引物对供试菌株扩增出550条带,其中多态性带175条,占总带数的31.8%。对供试菌株的AFLP指纹图谱聚类分析,可将其划分为FOGI、FOGII、FOGIII和FOGIV 4个类群(FOGs),分别为西瓜专化型菌株、冬瓜专化型菌株、黄瓜专化型菌株和甜瓜专化型菌株,聚类结果与菌株的专化型间存在明显的对应关系。Ten isolates of 4 gourd formae specialies which were Fusarium oxysporum f. sp. niveum, Fusarium oxysporum f. sp. benincasae, Fusarium oxysporum f. sp. cucumerinum and Fusarium oxysporum f. sp. melonis, were analyzed. The results showd that a total of the 550 AFLP bands were amplified using 10 pairs of primers, and 175 of the 550 bands were polymorphic, 31.8 % of them were polymorphisms. When all of polymorphic bands were analyzed by cluster analysis the 10 isolates were classified into 4 groups. There exist isolates of 4 different gourd forma specialies in FOGⅠ, FOGⅡ, FOGⅢ and FOGⅣ, respectively. There was evidently correspondent relationship between the cluster groups and formae specialies.

关 键 词：枯萎病菌 专化型 AFLP分析 

分 类 号：Q743[生物学—分子生物学]