心肌梗死患者细胞间黏附分子-1基因多态性研究  

Study on the intercellular adhesion molecule-1 polymorphisms in a Chinese population with myocardial infarction

作　　者：汪明[1] 李艳[1] 张平安[1] 杨超[1] 向萍霞[1] 韦叶生[2] 李晓艳[3] 黄从新[3] 

机构地区：[1]武汉大学人民医院检验科,430060 [2]广西壮族自治区右江民族医学院附属医院检验科 [3]武汉大学人民医院心内科

出　　处：《中华流行病学杂志》2005年第9期702-706,共5页Chinese Journal of Epidemiology

基　　金：湖北省自然科学基金资助项目(2003ABA183)

摘　　要：目的探讨汉族人群中细胞间黏附分子-1(ICAM-1)第4外显子+12959G/A、第6外显子+13848A/G基因多态性与心肌梗死(心梗)的相关性。方法提取165例心梗患者(包括急性心梗和陈旧性心梗)和199名健康对照者的DNA,采用序列特异性引物扩增法和聚合酶链反应-限制性片段长度多态性技术检测ICAM-1+12959G/A、+13848A/G基因多态性,同时测定血脂及血清超敏C反应蛋白(hsCRP,采用定量酶联免疫吸附试验技术)水平。结果湖北地区汉族人群中存在+13848A/G多态性,在心梗组中基因型分布为:AA型58.2%、AG型37.0%、GG型4.8%;对照组分别为45.7%、45.2%、9.0%,两组比较差异有统计学意义(P<0.05);基因型频率的相对风险分析发现,AA型患心梗的风险是GG型及AG型的1.651倍(OR=1.651,95%CI:1.089～2.504)。未检出+12959G/A多态性。结论ICAM-1+13848A/G基因多态性与湖北地区心梗易感性有关,+ 13848A等位基因可能是心梗发病的重要易感基因。Objective To investigate the association between intercellular adhesion molecule-1 （ICAM-1） gene ＋ 12959G/A and ＋ 13848A/G polymorphism and myocardial infarction （MI）, Methods Polymerase chain reaction-sequence specific primers（PCR-SSP） technology and PCR-restriction fragment length polymorphism（PCR-RFLP） were used for the detection of ICAM-1 genotypes in 165 patients with MI（including acute MI and old MI） and 199 healthy controls, Plasma lipid levels and hyper sensitive C reactive protein （quantitive ELISA method was used） levels were measured in all subjects, Results ＋ 12959G/A polymorphism was not detected in our study. The frequencies of AA,AG and GG Genotypes of ＋ 13848A/G were 58.2% and 45.7%,37.0% among patients and 45.2%,4.8% and 9.0% among controls,respectively, There were statistically significant differences in the distributions of the genotype frequencies（P〈 0.05） between two groups, and the relative risk suffered from MI of AA genotype was 1.651 times of the GG and GA genotype （ OR = 1.651,95 % CI ： 1.089-2.504 ）. Conclusion There was no ＋ 12959G/A polymorphism found in Chinese people, The coding single nucleotide polymorphism ＋ 13848A/G in the exon 6 of ICAM-1 gene was associated to MI and the allele A might serve as a risk factor for MI in Chinese.

关 键 词：心肌梗死 细胞间黏附分子 基因多态性 细胞间黏附分子- 心肌梗死患者 多态性研究 聚合酶链反应-限制性片段长度多态性 1基因 陈旧性心梗 超敏C-反应蛋白 

分 类 号：R542.22[医药卫生—心血管疾病]