油砂山油田本源微生物分子生物学初步调查  

Recent Investigation of Indigenous Microbial Molecular Research in Youshashan Oilfield

作　　者：佘跃惠[1] 何正国[2] 杨柳岸[3] 张凡[1] 周玲革[1] 舒福昌[1] 向廷生[1] 

机构地区：[1]长江大学化学与环境学院,湖北荆州434023 [2]湖北大学生命科学学院,湖北武汉430062 [3]青海油田分公司,甘肃敦煌736202

出　　处：《大庆石油地质与开发》2005年第5期82-83,共2页Petroleum Geology & Oilfield Development in Daqing

基　　金：湖北省科技厅国际合作重点项目(2004CA014)。

摘　　要：采用细菌通用引物和古细菌引物对青海油砂山油田178井本源微生物样品DNA进行扩增,构建基因文库,克隆和DNA序列分析。将测序结果与基因银行进行比对结果表明,油砂山样品中有大量海杆菌属和甲胞菌属,样品中还发现存在产甲烷古菌及一些不可培养的其它古菌类群。它们的功能和多样性有待于深入研究,以便进一步筛选用于提高该油田原油采收率的采油功能菌群。16SrDNA were amplified from DNA samples collected from Well 178 in Youshashan oilfield. These gene sequences were identified and classified based on clone library and sequence analysis. The results of blast in GeneBank indicated that the most of these 16SrDNA sequences were affiliated with Marinobacterium, and there were also Halomonas Methanolobus and uncultured archaeo in the samples. The functions and diversities of the bacteria need to be studied further for the application of improving oil recovery factor.

关 键 词：油砂山油田 本源微生物 基因文库 多样性 

分 类 号：TE357[石油与天然气工程—油气田开发工程]