裙带菜茎中硫酸多糖的分离纯化及分析鉴定  

Purification and Analysis of Poiysaccharides from the Caudex of Undaria pinnatifida

作　　者：康琰琰[1] 王一飞[1] 朱良[1] 门晓缓[1] 

机构地区：[1]暨南大学生物医药研究开发基地,广州510632

出　　处：《中药材》2005年第9期769-771,共3页Journal of Chinese Medicinal Materials

基　　金：广东省自然科学基金团队项目(粤科基办[2003]11号)

摘　　要：用热水从裙带菜茎中提取多糖,乙醇沉淀,Sevag法除蛋白,并采用DEAE-52和Sephadex G-200柱对其进行分离纯化,得到三种多糖纯品F_2、F_3和F_4,纯度鉴定为均一组分。紫外光谱显示不含蛋白质、多肽及核酸,通过硅胶G色谱分析,F_2、F_3和F_4中的单糖有半乳糖、岩藻糖、甘露糖、葡萄糖醛酸,F_2中除这四种单糖外,还含有葡萄糖和鼠李糖。To isolate polysaccharides with hot water from the caudex of Undaria pinnatifuta, and precipitate with ethanol. The protein in polysaccharides was removed by sevage way, DEAE-52 and Sephadex G-200 column chromatography were used to isolate and purification polysaccharides,three purified polysaccharides F2, F3 and F4 were obtained, It was identified that they were homogeneitv. The ultraviolet spectrum showed there was no proteins and nucleic acids in F2 ,F3 and F4. Through thin-layer chromatography analysis, F2, F3 and F4 were mainly composed of Gal, Fue, Man and Glu acid. F2 also contained Glu and Rha.

关 键 词：裙带菜 多糖 分离纯化 鉴定 硫酸多糖 纯度鉴定 SEPHADEX 葡萄糖醛酸 Sevag法 乙醇沉淀 

分 类 号：R282.77[医药卫生—中药学] R284.2[医药卫生—中医学]