注射用清开灵冻干粉解热镇痛作用及解热机制的试验研究  

Study on the Antipyretic, Analgesic Effects of Qingkailing Freeze-Dried Powder for Injection and Its Antipyretic Mechanism

作　　者：李东[1] 杨芳炬[1] 黄继华[1] 凌婧[1] 荆焰[1] 

机构地区：[1]四川大学华西基础医学与法医学院药理学教研室,成都610041

出　　处：《中药材》2005年第9期796-799,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:研究注射用清开灵冻干粉解热、镇痛作用及其解热机制。方法:建立家免2,4-二硝基苯酚及热模型及大鼠内毒素发热模型,观察清开灵冻干粉解热作用;采用小鼠扭体法及热板法,观察清开灵冻干粉镇痛作用。并用放免法检测大鼠下丘脑cAMP及IL-1β含量的变化。结果:注射用清开灵冻干粉能有效抑制内毒素导致的大鼠发热和2,4-二硝基苯酚导致的家兔发热,显著降低发热大鼠下丘脑IL-1β及cAMP的含量;明显减少醋酸致痛小鼠扭体次数,推迟热致痛小鼠添后足时间。结论:注射用清开灵冻干粉具有解热、镇痛作用,其解热机制可能通过抑制内生致热原IL-1β的生成,从而降低下丘脑发热介质cAMP的含量。Objective： To investigate the antipyretic, analgesic effects of Qingkailing Freeze-Dried Powder for Injection（QI） and its mechanism of the antipyretie effect. Methods： The rabbit model of 2,4-dinitrophenol-induced fever and the rat model of endotoxininduced fever were established and the body temperatures were observed. Radioimmunoassay was adopted to detect the levels of IL-1β and cAMP in the hypothalamus of rats. The analgesic effects were tested by hot-plate and writhing reaction method. Results： QI showed marked antipyretie effects on the 2.4-dinitrophenol-induced fever and the endotoxin-induced fever. At the same time, Q1 remarkably decreased the contents of IL-1β and cAMP in the hypothalamus. Q1 also remarkably reduced the frequency of acetic acid-induced writhing and delaied the time of licking rear feet. Conclusion： Q1 has antipyretic, analgesic effects. Its mechanism of the antipyretie effects on rats may be inhibiting the production of IL-1β and accordingly decreasing cAMP in the hypothalamus.

关 键 词：注射用清开灵冻干粉 解热 镇痛 IL-1Β CAMP 解热镇痛作用 解热机制 冻干粉 清开灵 注射用 

分 类 号：R971.1[医药卫生—药品] R285.5[医药卫生—药学]