银胶菊内酯对PGCL_3细胞uPA活性和表达的影响  

Effects of Parthenolide on the Activity and Expression of Urokinase Type Plasminogen Activator in PGCL_3 Cells

作　　者：钟东明 朱如芳 钟茂耀 

机构地区：[1]湛江市职业病防治所,广东湛江524018 [2]湛江市第三人民医院,广东湛江524001

出　　处：《中药材》2005年第9期800-802,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:研究银胶菊内酯对人肺癌细胞PGCL_3尿酶型纤溶酶原激沾物(uPA)蛋白表达及活性的影响, 探讨其抗肿瘤的作用机制。方法:MTT法检测银胶菊内酯对PGCL_3细胞的增殖抑制作用;发色底物法检测银胶菊内酯对PGCL_3细胞分泌的uPA活性的影响;Western blot法检测银胶菊内酯对uPA表达的影响。结果:5-160 μmol/L的银胶菊内酯作用PGCL_3细胞24 h后,明显抑制PGCL_3细胞的增殖,IC_(50)值为17.6μmol/L;5、10、20μmol/ L银胶菊内酯明显降低PGCL_3细胞分泌的uPA活性;银胶菊内酯还能降低PGCL_3细胞uPA蛋白表达。结论:银胶菊内酯抗肿瘤活性与uPA蛋白表达及活性有关。Objective：To study the effect of Parthenolide on the activity and expression of urokinase type plasminogen activator （uPA） on PGCL3 cells and to investigate the antitumor mechanisms of Parthenolide. Methods： MTT assay was used to determine the growth inhibition by Parthenolide in PGCL3 cells ;Effect of Parthenolide on the activity of uPA secreted by PGCL3 cells was measured by chromogenic substrate assay ;The expression level of uPA protein was assessed by Western blot analysis. Results ：Parthenolide could in- hibit proliferation of PGCL3 cells after 24h treatment, the IC50 value was 17.60 μmol/L; Parthenolide reduced significantly the activity of uPA secreted by PGCL3 cells and down-regulated the expression level of uPA protein. Conclusion：The antitumor activity of Parthenolide is involved in the activity and expression of urokinase type plasminogen activator.

关 键 词：银胶菊内酯 尿激酶型纤溶酶原激活物 肺癌 uPA活性 PGCL3 人肺癌细胞 MTT法检测 增殖抑制作用 mol/L WESTERN 

分 类 号：R285.1[医药卫生—中药学] R735.2[医药卫生—中医学]