胶州湾浮游植物遗传多样性及其季节变化研究  

Study on the genetic diversity and its seasonal variation of phytoplankton in the Jiaozhou Bay

作　　者：刘永健[1] 杨官品[1] 管晓菁[1] 门荣新[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《海洋学报》2005年第5期103-111,共9页

基　　金：国家自然科学基金资助项目(40176028).

摘　　要：分别于春、夏、秋三季提取了胶州湾浮游生物总DNA,用PCR方法扩增了浮游植物核酮糖1,5-二磷酸羧化/氧化酶大亚基(rbcL)基因片段,构建了三季节rbcL基因片段质粒文库.随机从各文库中选择约50个克隆,测定了这些克隆的序列.以氨基酸序列相同为标准,共获得59个分类操作单元(OTUs),其中春季特有的21个,夏季特有的13个,秋季特有的23个.有两个OTUs为春、夏季表层海水共有.基于OTU丰度计算的香农氏指数能反映遗传多样性水平.胶州湾春、夏、秋三季的香农氏指数分别为2.69,2.44和2.76,表明秋季胶州湾浮游植物遗传多样性最丰富.春、夏、秋三季浮游植物群落组成差异显著.除夏秋季有两个OTUs相同外,其他OTUs在季节间完全不同.表层海水浮游植物群落结构是动态的,随时间、空间的不同而变化.Ribulose-1, 5-bisphosphate carboxynase/oxygenase large subunit gene （rbcL） of phytoplankton DNAs of Jiaozhou Bay surface seawater was amplified in spring, summer and autumn and cloned respectively. One hundred and forty five clones about 50 each library were randomly selected and sequenced. If identical amino acid sequences are considered as the same operational taxonomy unit （OTU）, 59 OTUs are identified according to inferred amino acid sequences. Among these OTUs, 21 are specific for spring seawater, 13 are specific for summer seawater and 23 are specific for autumn seawater. Two OTUs are shared by summer and autumn. Shannon＇s index calculated from OTU abundances reflects the genetic diversity level of a community. The indexes of spring, summer and autumn surface seawater phytoplankton are 2.69, 2. 44 and 2. 76 respectively, indicating that phytoplankton genetic diversity of autumn seawater is the richest. Seasonal variation of phytoplankton community is significant; the community compositions of three seasons are almost completely different except for two OTUs shared by summer and autumn. Surface seawater phytoplankton communities are possiblv metacommunities different spatiallv and temporallv.

关 键 词：浮游植物 核酮糖1 5-二磷酸羧化 氧化酶大亚基基因 群落结构 季节变化 

分 类 号：Q178.53[生物学—水生生物学]