中华鳖种群RAPD分析  

RAPD Analysis of Genomic DNA in the Pelodiscus Sinensis

作　　者：肖亚梅[1] 陈丽莉[1] 陈合格[1] 赵如榕[1] 

机构地区：[1]湖南师范大学生命科学学院,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2005年第3期72-75,共4页Journal of Natural Science of Hunan Normal University

摘　　要：选用14个随机引物对中华鳖基因组DNA进行了随机扩增DNA片段多态性(RAPD)分析.14个引物,在8个待检样品中共扩增出714条清晰的DNA片段,片段大小在200～3 000bp之间,平均每一引物在每个样品中扩增了6.0条带.统计结果表明,中华鳖群体内平均相似率0.857,总平均带纹频率0.773,遗传变异率为0.227.研究表明,中华鳖为遗传共享度较高的群体.The genomic DNA of the Pelodiscus sinensis were ampilified with 14 random primers, then 714 bands were observed with 14 primers in 8 samples. The length of these DNA fragments were varied from 200 to 3 000 bp. On average, there existed about 6.0 bands being observed with single primer and each sample. The intra-population band sharing was 0. 857, average band frequency was 0. 773, and the genetic variability was 0. 227.

关 键 词：中华鳖 RAPD 遗传变异率 

分 类 号：Q959.6[生物学—动物学]