反相高效液相色谱法测定人全血中吲达帕胺浓度  

RP-HPLC Determination of Indapamide in Human Whole Blood

作　　者：陈钧[1] 梅妮[1] 谢月玲[1] 江文明[1] 蒋新国[1] 

机构地区：[1]复旦大学药学院药剂学教研室,上海200032

出　　处：《药物分析杂志》2005年第9期1035-1037,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立测定人全血中吲达帕胺的反相高效液相色谱法。方法:采用 Dikma Diamonsil C_(18)分析柱(200 mm×4.6 mm,5μm),以0.1%三乙胺缓冲液(用磷酸调pH=3.75)-乙腈(65:35)为流动相,流速1.0 mL·min^(-1),检测波长240 nm,柱温40℃。以卡马西平作内标,血样用乙醚提取2次。结果:本方法线性范围为5～1000 ng·mL^(-1),r=0.9999,方法回收率在94%～103%之间,日内和日间 RSD 均小于7%。结论:本方法灵敏,准确度、重现性好,适用于吲达帕胺的药代动力学和生物利用度研究。Objective： An RP - HPLC method was developed for the determination of indapamide in human blood. Method：The chromatographic separation was carried out on Dikma Diamonsil C18 column（200mm×4.6mm,5μm）with the mobile phase consisting of 0.1% triethylamine buffer（ adjusted pH to 3.75 with phosphoric acid） - acetonitrile （65： 35 ）, flow rate at 1.0 mL·min^-1. Detection wavelength was set at 240 nm and column temperature was 40℃. Carbamazepine was used as the internal standard and blood samples were extracted by ether. Result：The linear range was 5 - 1000 ng·mL^-1,r =0. 9999. The method recovery was within 94% - 103%. Within - day and between -day RSD were less than 7%. Conclusion： The method is sensitive, specific and reproducible for the determination of indapamide concentration in human blood. It is very suitable for pharmacokinetics and bioavailability study of indapamide.

关 键 词：吲达帕胺 血药浓度 高效液相色谱法 反相高效液相色谱法 人全血 测定 生物利用度研究 浓度 C18分析柱 药代动力学 

分 类 号：R927.2[医药卫生—药学] R972.4