川西獐牙菜中不同部位3种药用成分的反相高效液相色谱测定  

RP-HPLC Determination of Three Medicinal Bioactive Components in Different Parts of Swertia mussotii Franch

作　　者：马玉花[1] 陈桂琛[1] 吉文鹤[1] 卢学峰[1] 纪兰菊[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810001

出　　处：《药物分析杂志》2005年第9期1079-1081,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家中西部重点项目(2001BA901A47)

摘　　要：目的:建立野生与栽培的川西獐牙菜中不同部位3种有效成分,即龙胆苦苷(S_1)、芒果苷(S_2)、7-O-β-D-吡喃木糖-1,8-二羟基-3-甲氧基(口山)酮(S_3)的反相高效液相色谱定量分析方法。方法:色谱柱为 Kromasil C_(18)(250mm×4.60 mm,5μm),流动相为甲醇-0.02%磷酸水溶液,梯度洗脱,洗脱条件为0 min→50 min→55 min,甲醇:20%→80%→100%;流速为1 mL·min^(-1),检测波长为260nm。结果:该方法具有很好的线性关系和回收率。结论:本方法简单、准确、快速、实用性强。川西獐牙菜中3种药效成分在花的部位含量最高。gentiopicroside（ S1 ）, mangiferin （ S2 ） and 7 - O -β - D - xylopyranosyl - 1,8 - dihydroxy - 3 - methoxyxanthone （ S3 ） in different parts of wild and cultivated Swertia mussotii Franch were determined by RPHPLC. Methods：The analysis was performed on a Kromasil C18 （250mm×4.60mm,5μm）. The mobile phase consisted of CH3OH and 0. 02% aqueous phosphoric acid. The gradient condition was 0 min→50 min→55 min, CH3OH 20%→80%→100%. The flow rate was 1 mL·min^-1. The detection wavelength was at 260 nm. Results： The good linear relationships and recoveries were obtained under the optimum conditions for every component. Conelusion：The method is simple,rapid and reliable. The flower of this herb contains more three medicinal bioactive components than its other parts do.

关 键 词：金川西獐牙菜 不同部位 龙胆苦苷(S1) 芒果苷(S2) 7-O-β-D-吡喃木糖-1 8-二羟基-3-甲氧基山酮(S3) 反相高效液相色谱法 反相高效液相色谱 川西獐牙菜 高效液相色谱测定 药用成分 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]