铜(Ⅱ)-苏氨酸-邻菲咯啉配合物的合成及其与DNA作用的光谱研究  

Synthesis and DNA Binding Spectroscopy Studies of Cu(Ⅱ)-Thr-Phen

作　　者：张芳[1] 张前前[1] 祝陈坚[1] 王修林[1] 

机构地区：[1]中国海洋大学化学化工学院,山东青岛266003

出　　处：《光谱学与光谱分析》2005年第9期1439-1442,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金(20371043)资助项目

摘　　要：合成了一种未见报道的铜(Ⅱ)苏氨酸邻菲咯啉配合物。根据元素分析、红外光谱、差热热重分析对其进行表征;并以电子吸收光谱法及溴化乙锭(EB)荧光分析法研究了此种配合物与DNA的作用。结果表明:此种配合物与DNA作用时,其电子吸收光谱的最大吸收峰明显红移,并产生明显的减色效应,同时,配合物也能较大程度地猝灭EBDNA体系的荧光,表明此种配合物与DNA存在很明显的插入结合作用。A new complex [ Cu （Thr） （Phen）H2O]SO4· H2O· CH3OH （Thr = DL-Threonine, Phen = o-Phenanthroline）, which has not been published, was synthesized and characterized by elemental analysis, IR spectroscopy, and TG-DTA. The interaction of the complex and sperm DNA was studied by electronic absorption and ethidiurn bromide （EB） fluorescence spectroscopy. The result indicates that the maximal absorption peaks of this complex are red-shifted and the intensity is weakened; At the same time, it can to some extent quench the emission intensity of EB-DNA system. Therefore, the authors come to a conclusion that the interaction of this complex and sperm DNA is intercalation.

关 键 词：铜(Ⅱ)-苏氨酸-邻菲咯啉配合物 鱼精DNA 插入结合 

分 类 号：O614.1[理学—无机化学]