锌(Ⅱ)-氟罗沙星光化学荧光法测定药物制剂中的氟罗沙星  

Determination of Fleroxacin by Photochemical Fluorescence of Zinc-Fleroxacin Complex

作　　者：冯瑞琴[1] 丁芬[1] 刘昱[1] 

机构地区：[1]北京师范大学化学系,北京100875

出　　处：《光谱学与光谱分析》2005年第9期1468-1470,共3页Spectroscopy and Spectral Analysis

基　　金：北京市自然科学基金(2022007)资助项目

摘　　要：氟罗沙星在紫外光的照射下易发生光解作用。依据氟罗沙星的光解产物与Zn2+离子形成配合物,能显著地增敏氟罗沙星的荧光强度,建立了敏化荧光分析氟罗沙星的新方法。探讨了酸度、[Zn2+]/[PHFLRX]浓度比和光照等对荧光强度的影响。结果表明该方法的线性范围为5.0×10-8～5.0×10-6mol·L-1,检出限为4.2×10-8mol·L-1。对浓度为5.0×10-7mol·L-1的氟罗沙星平行测定20次,计算其相对标准偏差(RSD)为1.7%。对针剂、片剂和尿样中的氟罗沙星分别进行了测定,其回收率为95.0%～105.0%。并在实验的基础上,对其机理进行了合理的推测。Based on the complex formed by Zn^2 ＋ which can strengthen the relative fluorescence intensity of fleroxacin evidently, a novel photochemical fluorescence method was developed. The effects of the acidity, the concentration ratio of Zn^2＋ to fleroxacin, and the time for illumination were studied. Under the optimum experiment conditions, the linear range of the determination was 5.00 × 10^-8-5.00× 10^-6 mol·L^-1. The detection limit was 4.2× 10^-8 mol· L^-1. The relative standard deviation of the determination of fleroxacin （5.0 × 10^-7 mol·L^-1） was 1.7% （ n = 20）. The method was successfully applied to the determination of fleroxacin in specimens, and the recoveries were in the range of 95.0%-105%. The mechanism of this system is proposed.

关 键 词：氟罗沙星 锌离子 光化学荧光 

分 类 号：O657.3[理学—分析化学]