生长激素对大鼠阿霉素心肌病心肌细胞凋亡及Fas的影响  

EFFECTS OF GROWTH HORMONE ON CARDIOMYOCYTE APOPTOSIS IN ADRIAMY-CIN-INDUCED CARDIOMYOPATHY IN RATS

作　　者：姚丽梅[1] 陈宇明[1] 尹瑞兴[2] 

机构地区：[1]广西医科大学第一附属医院老年心内科,南宁530021 [2]广西医科大学科研处

出　　处：《广西医科大学学报》2005年第4期509-511,共3页Journal of Guangxi Medical University

基　　金：广西壮族自治区自然科学基金项目资助(桂科基0448059)

摘　　要：目的:观察生长激素(Growthhormone,GH)对阿霉素(Adriamycin,ADR)心肌病大鼠心肌细胞凋亡的影响及凋亡相关基因Fas蛋白表达的影响。方法:30只雄性Wistar大鼠,体重200～250g,随机分为3组:对照组、ADR组和GH+ADR组。用原位末端标记法(TUNEL)标记凋亡的心肌细胞,用免疫组织化学方法检测Fas蛋白的表达。结果:与对照组比较,ADR组心肌细胞凋亡指数明显升高(P<0.05),GH+ADR组细胞凋亡指数低于ADR组(P<0.05)。ADR组Fas蛋白表达明显高于对照组(P<0.05)。GH+ADR组Fas蛋白表达明显低于ADR组(P<0.05)。结论:心肌细胞凋亡是阿霉素心肌病的重要发病机制,Fas蛋白表达升高是诱发凋亡的机制之一。GH干预治疗可减少阿霉素心肌病的心肌细胞凋亡,Fas基因蛋白表达的减少是其抑制凋亡的可能机制之一。Objective：To explore the effects of growth hormone （GH） on cardiomyocyte apoptosis and the expression of Fas protein in adriamycin （ADR）-induced cardiomyopathy in rats. Methods： 30 male Wistar rats weighing 200-250 g were randomized into three groups： control group, GH＋ADR group and ADR group. Apoptotic cardiomyocytes were detected by using the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling method （TUNEL）. The expressionion of Fas proteins in myocardium was determined by immunohistochemical method. Result：As compared with the control animals, ADR group had significantly higher index of apoptotic cardiomyocytes （P 〈0.05）. The number of apoptotic cardiomyocytes in GH＋ADR group was less than that in ADR group （ P 〈0. 05）. The expression of Fas protein in ADR group was significantly higher than that in control group （ P 〈0. 05）. The expression of Fas protein in GH＋ ADR group was significantly lower than that in ADR group control group （P 〈 0. 05）. Conclusion： Myocardial apoptosis is an important reason of adriamycin-induced cardiomyopathy. One of the mechanisms might be that ADR can increase Fas protein expressionion. GH therapy can effectively inhibit cardiomyocyte apoptosis in adriamycin-induced cardiomyopathy, partly because it might decrease the expression of Fas protein.

关 键 词：生长激素 阿霉素 心肌 凋亡 大鼠 

分 类 号：R542[医药卫生—心血管疾病]