三种脾虚证模型小鼠消化吸收功能改变的比较研究  

Comparative Study on Changes of Digestive and Absorptive Functions in Three Different Models of Pi-Deficiency Syndrome

作　　者：胡琳琳[1] 高云芳[1] 何志仙[1] 

机构地区：[1]西北大学生命科学学院,西安710069

出　　处：《中国中西医结合杂志》2005年第9期813-816,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金(No.30370202);陕西省自然科学基金(No.DH98273);陕西省教育厅重点科研基金(No.00JK009)

摘　　要：目的对3种脾虚证模型小鼠在消化吸收方面的改变进行比较。方法实验动物分为4组,即对照组、大黄组、力竭游泳组和劳倦过度组。观察各组小鼠一般体征、D木糖排泄率、血清淀粉酶活性、胃排空、肠推进速度、血清胃泌素含量、脏器指数等指标的变化。结果3种方法塑造的脾虚小鼠模型均出现了D木糖排泄率降低、胃排空、肠推进速度加快。与对照组比较,劳倦过度组的所有指标均有显著变化,而力竭游泳组的血清淀粉酶活性、脾脏指数降低不显著,大黄组的血清胃泌素含量变化、胸腺指数变化不显著。结论劳倦过度法塑造脾虚证动物模型各指标的变化显著,大黄泻下法和力竭游泳法变化不显著。Objective To compare the changes of digestive and absorptive functions in three different models of Pi-deficiency syndrome （PDS）. Methods Experimental mice were divided into four groups, the control group（CG）, the rhubarb group （RG）, the exhaustion group（EG） and the over-exertion group（OG）. Criteria including general physical signs, D-xylose excretion rate, serum amylase activity,velocity of stomach emptying and enterokinesia, serum gastrin content and indexes of organs were determined before and after treatment. Resuits Decrease of D-xylose excretion rate and increase of stomach emptying and enterokinesia velocity appeared in all the three PDS models. As compared with CG, changes of all indices in OG were significant, while the decreasing of spleen index and serum amylase activity in EG, and the changes of serum gastrin content and thymus index in RG were insignificantly different. Conclusion All the changes in various criteria showed that PDS mice model established by over-exertion was superior to that established by frequently used methods as purging with rhubarb and exhausting by swimming.

关 键 词：脾虚证 动物模型 消化吸收功能 模型小鼠 消化吸收 功能改变 D-木糖排泄率 血清淀粉酶活性 血清胃泌素 劳倦过度 

分 类 号：R256.3[医药卫生—中医内科学]