酸性刺激对大鼠骨骼肌细胞胞浆Ca^(2+)的影响  

Research on Effects of Acid on Cytoplasmic Ca^(2+) Metabolism of Rat Skeletal Muscles

作　　者：周锦琳[1] 田野[2] 

机构地区：[1]北京体育大学,北京100084 [2]国家体育总局体育科学研究所,北京100061

出　　处：《北京体育大学学报》2005年第9期1201-1203,1232,共4页Journal of Beijing Sport University

基　　金：北京体育大学博士学位论文;教育部博士点基金课题。

摘　　要：研究表明,运动引起胞浆Ca2+聚积是骨骼肌疲劳的重要原因。从研究酸性刺激时胞浆Ca2+的变化入手,从细胞水平分析急性运动可能对骨骼肌胞浆钙稳态的影响,试图为全面了解运动性疲劳的产生机制提供理论依据。实验中以Fluo-3-AM为Ca2+荧光指示剂,通过激光扫描共聚焦显微镜系统对用体外组织分离和培养的方法获得的单个的骨骼肌细胞胞浆Ca2+的荧光强度及其动态变化进行观察。结果发现:骨骼肌细胞受到酸性刺激时,胞浆Ca2+稳态被破坏。其总体变化趋势是先略有增加后迅速减少,直至在比正常低的水平上形成新的动态平衡。钙波的变化既有振幅的变化,也有频率的变化。这可能提示:运动后,由于内环境酸性代谢产物的堆积,致使胞浆Ca2+稳态破坏,最终引起骨骼肌疲劳。A growing body of hterature indicated that the exercise resulted in the decrease of Ca^2＋ transportation capacities. Cytoplasmic Ca^2＋. accumulation can be seen as an important factor that caused skeletal muscle fatigue. Consequently, a systematic study was conducted to observe the effect of acid on cytoplasmic Ca^2＋ in order to analyze how acute exercise influenced the cytoplasmic Ca^2＋ homeostasis. The fluorescent indicator, Fluo - 3 - AM and the laser confocal microscope system were used for measurements of cytoplasmic Ca^2＋ concentration and its time course of individual skeletal muscle cells, which were obtained by the isolation culture techniques. This study was conducted at the cell level to explore the mechanisms of exercise-induced fatigue, which may provide scientific training with theories foundation. The results indicat- ed that the cytoplasmic Ca^2＋ homeostasis was disrupted when the skeletal muscle cells were in the acid condition（pH = 6. 9）. Cytoplasmic Ca^2＋ initially increased, then decreased, and in the end came into being a new Ca^2＋ homeostasis under the base hne. The swing as well as the frequency of cytoplasmic Ca^2＋ wave changed while muscle cells were in acid. It suggested cytoplasmic Ca^2＋ homeostasis disruption due to the accumulation of acidic metabolic products resulted in the muscle fatigue after acute exercise.

关 键 词：骨骼肌疲劳 酸性刺激 钙稳态 胞浆Ca^2+ 

分 类 号：G804.2[文化科学—运动人体科学]