胡萝卜发根培养甘薯茎线虫体系研究  

Study of monoxenic culture of the Ditylenchus destructor on carrot hairy roots

作　　者：肖翔 [1] 阮龙 [1] 王钰 [2] 潘燕 [1] 余增亮 [1] 吴跃进 [1] 许安 [1] 

机构地区：[1]中国科学院等离子体物理研究所,离子束生物工程学重点实验室,合肥,230031 [2]安徽大学生命科学院,合肥,230031

出　　处：《高技术通讯》2005年第9期67-70,共4页Chinese High Technology Letters

基　　金：国家高技术研究发展计划(863计划)

摘　　要：利用发根农杆菌转化的胡萝卜发根建立了甘薯茎线虫的单寄主培养体系,观察了甘薯茎线虫的行为及生活史.结果表明:在胡萝卜发根上培养甘薯茎线虫,便于研究其行为,可以清楚地观察到线虫在根毛处自由取食及侵入发根内部活动情况;雌虫可以正常产卵,线虫在20～25天内完成正常的生活史.基于观察到的上述结果,初步证实了所构建的胡萝卜发根培养甘薯茎线虫体系的可行性.The monoxenic culture of Ditylenchus destructor on Agrobacterium rhizogenes transformed carrot hairy roots was successfully established. The D. destructor＇s behavior and complete life cycle were observed in vitro. The results showed this culture system could help study the proceeding of D. destructor invading the carrot roots; The feeding, reproduction and movements of D. destructor were observed successively in the mot tissue. The female D. destructor could lay eggs normally and the whole life cycle needed about 20 - 25 days. So it is feasible to produce and study the endoparasitic nematodes D. destructor in this monoxenic culture.

关 键 词：甘薯茎线虫 发根农杆菌 单寄主培养 胡萝卜发根 甘薯 发根培养 胡萝卜 体系研究 农杆菌转化 培养体系 活动情况 生活史 寄主 

分 类 号：S435.315.9[农业科学—农业昆虫与害虫防治] Q946.8[农业科学—植物保护]