缝隙连接蛋白重构对肥厚心室肌心电生理的影响  

Influence of connexin recombination on electrophysiology of hypertrophic myocardium

作　　者：陈振光[1] 罗红鹤[1] 陈向来[1] 钟佛添[1] 张希[1] 孙培吾[1] 

机构地区：[1]中山大学附属第一医院心胸外科,广州510080

出　　处：《广东医学》2005年第10期1341-1343,共3页Guangdong Medical Journal

基　　金：广东省自然科学研究基金资助项目(编号:940047)广东省医学科研基金资助项目(编号:A2003161)

摘　　要：目的观察心脏缝隙连接蛋白(Cx43)重构对肥厚心室肌心电生理的影响。方法随机选用新西兰白兔20只分为A(正常心肌)和B(肥厚心肌)两组,采用40%-60%结扎腹主动脉后饲养建立兔肥厚心肌模型,BL -410生物机能实验系统记录校正Q—T间期和R波振幅,切取左心室肌采用免疫组化测定缝隙连接蛋白Cx43 荧光表达面积和强度。结果B组心脏重量/体重比、左心室重量/体重比较A组明显增加,动物模型建立成功。校正Q—T间期B组[(332.57±24.34)ms]明显长于A组[(309.86±12.13)ms],上升约7.3%,R波振幅B组[(502.00±22.13)μm]明显高于A组[(426.29±33.96)μm]。Cx43荧光表达面积B组[(4232.33±484.43)μm2]明显于A组[(5 325.62±598.90)μm2],下降约20.5%,与校正Q—T间期相关(r=-0.775),并存在侧边偏移现象。结论肥厚心室肌存在缝隙连接蛋白Cx43数量下降和位置异常的重构现象,可能是肥厚心肌对缺血耐受性差、容易出现心律失常的分子病理学基础。Objective To study the influence of eonnexin 43（Cx43） recombination on electrophysiology of hypertrophic myocardium. Methods 20 New Zealand rabbits were randomly divided into group A （normal） and group B （hypertrophic myocardium）. Abdominal aorta were straiten 40% - 60% in diameter to promote myocardial hypertrophy. QT interval and R wave range were estimated with BL- 410 electrocardiogram. Expression of Cx43 was examined by immtmehistochemistry. Results QT interval was longer in group B[ （332.57 ± 24.34）ms] than in group A[ （309.86 ± 12.13）ms] and R wave range was higher in group B [ （502.00 ± 22.13）μm]than in group A [ （426.29 ± 33.96）μm]. Area of connexin43 expression was higher in group A [（5 325.62 ± 598.90）μm^2 ]than in group B [（4 232.33±484.43）μm^2] and was correlated to QT interval （r = - 0.775）. Density of Cx43 expression was not significantly different between two groups. Conclusions There is Cx43 recombination in hypertrophic myocardium including significant decrease and translocation. It may be the molecular pathological change to development of arhyhmwa during ischemia.

关 键 词：心脏肥厚 缝隙连接蛋白 心电生理 兔 肥厚心肌 左心室肌 BL-410生物机能实验系统 连接蛋白CX43 Q-T间期 新西兰白兔 

分 类 号：R541.75[医药卫生—心血管疾病] R542.2[医药卫生—内科学]