丹那唑治疗子宫内膜异位症作用机制的研究  

The study on treatment of endometriosis with Danazol

作　　者：郑辉[1] 马保锋[1] 潘丽[1] 黄以萍[1] 

机构地区：[1]暨南大学医学院,510632

出　　处：《中国妇幼保健》2005年第19期2476-2478,共3页Maternal and Child Health Care of China

摘　　要：目的:探讨丹那唑治疗子宫内膜异位症(内异症)的作用机制。方法:建立内异症大鼠模型,丹那唑组(n=29)分别皮下注射丹那唑2、4、6、8周,对照组(n=6)皮下注射生理盐水,观察丹那唑对异位内膜生长情况的影响。取丹那唑治疗8周组和对照组的异位内膜,用逆转录聚合酶链反应(RT-PCR)技术,检测丹那唑治疗内异症对异位内膜白细胞介素-6(IL-6)mRNA表达的影响。结果:建模后第3周异位内膜生长良好,各组移植物体积无明显差异(P>0.05)。用丹那唑分别治疗2、4、6和8周后,异位内膜体积缩小(P<0.01),并且用药4、6和8周与用药2周相比,作用更明显(P<0.01)。形态学检查显示,用丹那唑治疗2周后,异位内膜无明显增生;治疗4、6和8周后,异位内膜呈萎缩状态。大鼠成模后给予丹那唑治疗8周,异位内膜IL-6 mRNA的表达明显减少,与对照组相比差异有高度显著性意义(P<0.01)。结论:丹那唑治疗可使内异症大鼠异位内膜逐渐萎缩退化,其作用与用药时间长短有关;丹那唑治疗可使内异症大鼠异位内膜IL-6 mRNA的表达明显降低。Objective： To investigate the effects of Danazol on ectopic endometfium and the effect of Danazol on expression of IL - 6 mRNA in the ectopic endometrium. Methods： To establish the rat models, Danazol was administered repeatly as a s. c. injection per day separately for 2, 4 , 6 and 8 weeks （ n = 29 ）. The control groups （ n = 6） were injected s. c. with normal saline. After treatment, the laparotomy was performed again to investigate the state of the endometfial explants. Endometfiotic tissue was cut from group treated with Danazol for eight weeks and control group. IL - 6 mRNA expression in the ectopic endometrium was examined by reverse transcription - polymerase chain reaction （RT-PCR）. Results： The 3rd week after modelling , no significant difference was noted for the size of explants between groups （ P 〉 0. 05 ）. After treatment with Danazol separately for 2, 4, 6, and 8 weeks, the volume of endometrial explants was reduced significantly （P 〈 0. 01 ） , especially 4, 6, 8 -week administered groups （P 〈 0. 01 ）. The 2nd week after treatment, ectopic endometrium was not further proliferated and the 4th, 6th, 8th weeks after treatment, the tissue became atrophy. 8 weeks after treatment, the level of IL -6 mRNA reduced significantly compared with that of control group （P 〈 0. 01 ）. Conclusion： Danazol treatment can induce ectopic endometfium atrophy gradually; its effect correlates with treatment duration. Danazol treatment can decrease the level of IL - 6 mRNA in endometriotic tissue significantly.

关 键 词：丹那唑 子宫内膜异位症 异位内膜 白细胞介素-6 

分 类 号：R711.71[医药卫生—妇产科学]