一种新型超临界流体萃取-高效液相色谱在线联用系统的构建  

Establishment of a New On-line Coupled Supercritical Fluid Extraction(SFE) with High Performance Liquid Chromatography(HPLC) System

作　　者：张洁[1] 段继诚[1] 梁振[1] 张丽华[1] 张维冰[1] 霍玉书[2] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心,大连116023 [2]德克萨斯大学健康科学中心,圣安东尼奥782293900

出　　处：《高等学校化学学报》2005年第10期1832-1834,共3页Chemical Journal of Chinese Universities

基　　金：中国科学院知识创新工程重要方向性项目(批准号:KGCX2-SW-213)资助.

摘　　要：A new on-line coupled supercritical fluid extraction(SFE) with high performance liquid chromatography(HPLC) system was developed.Two three-port valves were used as an interface to connect SFE and HPLC.In this design,the two important processes including sample preparation and sample analysis were(integrated) into a system.Compared with off-line system,the on-line system has many advantages,such as high sensitivity,short analysis time,avoiding sample pollution,easily operation and ease to automation etc.Its application in the extraction and analysis of triterpenoids to the fruiting bodies of Ganoderma Lucidum was also described.Under the optimized extraction conditions,the similar chromatogram of the extract with on-line SFE- HPLC system was observed as well as that with off-line SFE followed by HPLC.The result showed the great potential of the on-line system in the extraction and analysis of tradition Chinese medicine.A new on-line coupled supercritical fluid extraction（SFE） with high performance liquid chromatography（HPLC） system was developed. Two three-port valves were used as an interface to connect SFE and HPLC. In this design, the two important processes including sample preparation and sample analysis were integrated into a system. Compared with off-line system, the on-line system has many advantages, such as high sensitivity, short analysis time, avoiding sample pollution, easily operation and ease to automation etc. Its application in the extraction and analysis of triterpenoids to the fruiting bodies of Ganoderma Lucidum was also described. Under the optimized extraction conditions, the similar chromatogram of the extract with on-line SFE- HPLC system was observed as well as that with off-line SFE followed by HPLC. The result showed the great potential of the on-line system in the extraction and analysis of tradition Chinese medicine.

关 键 词：在线联用系统 超临界流体萃取 高效液相色谱 灵芝 

分 类 号：O658[理学—分析化学]