结核患者Th1/Th2细胞因子和前炎性因子研究  

Study of Th1/Th2 cytokines and pro-inflammatorycytokines in tuberculosis patients

作　　者：张国龙[1] 光山正雄[2] 杜长梅[1] 石瑞如[1] 王江东[1] 高飞絮[1] 张霞[1] 

机构地区：[1]河南省胸科医院,河南郑州450003 [2]日本京都大学医学部

出　　处：《山东医药》2005年第28期11-12,共2页Shandong Medical Journal

基　　金：日美医学协会结核麻风委员会基金资助项目;河南省医学科技创新人才工程项目(2002112)

摘　　要：目的研究结核患者细胞因子变化规律和调控机制,以帮助临床鉴别诊断。方法对54例结核患者、18例肺癌患者和12例健康人血清、肺泡灌洗液、胸水及脑脊液等多种标本的各种细胞因子进行检测。结果结核患者血清白介素(IL)-12、IL-18等T h1细胞因子及IL-10、IL-4等T h2细胞因子水平均较高。各种细胞因子在脑脊液中含量均较低;干扰素γ(IFN-γ)在胸水渗出液中含量很高。前炎性因子IL-6、IL-8和肿瘤坏死因子-α(TNF-α)在结核患者中水平较高。IL-10与TNF-α的比值在结核和肿瘤患者之间有显著差异。结论细胞因子检测有助于结核病和肺癌患者的鉴别诊断。Objective： To determine different cytokine levels in different samples of tuberculosis cases, Methods： Sera. pleural fluid, BALF and CSF were obtained from a total of ,54 tuberculosis cases, 18 lung cancer cases, and 12 healthy individuals. Different cytokines including Thl cytokines （IL-12, IL-18 and IFN-γ）. Th2 cytokines （IL-4 and IL-10）. pro-inflammatory cytokines （IL-6, IL-8. and TNF-α） and some cytokine receptors （IFN-γR and IL-12R） were detected. Results： IL-12,IL-18, IL-10 and IL-4 showed higher levels in tuberculosis patients. The levels of most cytokines in CSF were very low. but a high level of IFN-γ was observed in pleural fluid. The pro-inflammatory cytokines, IL-6, IL-8 and TNF-α. were detected at a higher level in tuberculosis cases. Ratio of IL-10 and TNF-α had a significant difference between tuberculosis and lung cancer cases. Conclusion： Cytokines detection can be used for a tuberculosis and lung cancer differential diagnosis.

关 键 词：结核 细胞因子 前炎性因子 鉴别诊断 

分 类 号：R521[医药卫生—内科学]