毛细管区带电泳法拆分盐酸舍曲林对映体  

Separation of cis-trans Isomers and Enantiomers of Sertraline Hydrochloride by Capillary Zone Electrophoresis

作　　者：陈德英[1] 陈玉英[1] 戴荣继[2] 胡育筑[1] 

机构地区：[1]中国药科大学基础部,南京210009 [2]北京理工大学生命科学与技术学院,北京100081

出　　处：《中国药科大学学报》2005年第5期440-443,共4页Journal of China Pharmaceutical University

摘　　要：目的:研究影响盐酸舍曲林对映异构体分离的因素,比较不同种类环糊精对盐酸舍曲林对映异构体的手性选择性,建立拆分盐酸舍曲林对映异构体的毛细管区带电泳分离方法。方法:以15 mg/mL羧甲基-β-环糊精(CM--βCD)为手性添加剂,采用30 mmol/L四硼酸钠(pH 11)为背景电解质,操作电压为15 kV,柱温为20℃,波长为210 nm的条件下,分离了盐酸舍曲林对映异构体。结果:盐酸舍曲林对映异构体在15 min内达到基线分离。结论:阴离子环糊精对盐酸舍曲林对映异构体的手性选择性比中性环糊精高。AIM：To study the factors influencing the separation of cis-trans isomers and enantiomers of sertraline hydrochloride, particularly the influences of different types of cyclodextrins （CDs） on the chiral selectivity, and to establish the method of for the separation of cis-trans isomers and enantiomers of sertraline hydrochloride by capillary zone electrophoresis （CZE）. METHODS： Optimal separation was achieved using a buffer （pH 11 ） of 30 mmol/L sodium borate containing 15 mg/mL CM-β-CD;The voltage and temperature was 15 kV and 20 ℃, respectively;The wavelength of 210 nm was used for detection. RESULTS： Baseline resolution of cis-trans isomers and enantiomers of sertraline hydrochloride was reached in 15 nm min. CONCLUSION：The chiral selectivity of negatively charged CDs is better than that of the neutral CDs.

关 键 词：毛细管区带电泳 盐酸舍曲林 对映异构体 环糊精 手性分离 

分 类 号：O658.9[理学—分析化学] TQ463[理学—化学]