大鼠异位心脏移植模型的术式探讨  

Procedures of heterotopic heart transplantation in rats

作　　者：马毅[1] 陈细桃[2] 朱晓峰[1] 何晓顺[1] 陈规划[1] 

机构地区：[1]中山大学附属第一医院器官移植中心,广州510080 [2]中山大学医学实验动物中心

出　　处：《中华实验外科杂志》2005年第10期1238-1240,i0002,共4页Chinese Journal of Experimental Surgery

基　　金：广东省自然科学基金项目(05300768)

摘　　要：目的探讨大鼠异位心脏移植模型的手术操作方法,并对大鼠颈部和腹部异位心脏移植的术式进行了比较。方法施行颈部异位心脏移植60次和腹部异位心脏移植90次,均采用显微外科缝合法进行动静脉吻合。结果颈部和腹部异位心脏移植供心获取时间分别为(10.0±2.2)min和(7.2±0.8)min。受体血管吻合时间分别为(28.2±5.6)min和(20.6±3.8)min。无干预同系移植组中,颈部和腹部异位心脏移植手术成功率分别为83.2%和92.6%,两种术式的手术成功率差异无统计学意义(P>0.05)。结论两种异位心脏移植术式均可作为移植免疫及药物实验研究的可靠动物模型,两种术式分别有其各自的优缺点,术式选择主要根据实验者的设计进行。Objective To explore the surgical techniques of heterotopic heart transplantation in rats and investigate the difference between cervical heart transplantation and abdominal heart transplantation. Methods Sixty cases of heterotopic cervical heart transplantation and 90 cases of heterotopic abdominal heart transplantation were performed, and vessels anastomosis carried out by micro-surgical techniques. Results Donor heart harvesting time in cervical and abdominal groups was （10.0 ± 2.2） min and （7.2 ± 0.8） min respectively, and the vessels anastomosis time in recipients was （28.2 ± 5.6） min and （20.6 ± 3.8） min respectively. In isograft transplantation without any preconditioning, operative successful rate was 83.2 % and 92.6 % in cervical and abdominal group respectively with the difference being not significant between two groups （ P 〉 0.05）. Conclusion Two kinds of heterotopic heart transplantation methods are stable models for research of transplant immune and drug experiments, since they have their advantages and disadvantages. The designers can choose the method according to the requirements of the research.

关 键 词：大鼠 异位心脏移植手术 动物模型 移植免疫 血管吻合 

分 类 号：R654.2[医药卫生—外科学] R-332[医药卫生—临床医学]