黄蘑多糖Fb对小鼠免疫功能的调节作用  

Regulatory effects of polysaccharide Fb from Huangmo on immunological function in mice

作　　者：马岩[1] 马颖哲[1] 张家颖[2] 李晓梅[2] 

机构地区：[1]吉林大学基础医学院生物化学与分子生物学教研室,吉林长春130021 [2]吉林大学基础医学院生物化学与分子生物学实验中心,吉林长春130021

出　　处：《吉林大学学报（医学版）》2005年第5期692-695,共4页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅社会发展基金课题资助(20020803)

摘　　要：目的:研究黄蘑多糖Fb(polysaccharid Fb from Huangmo)对小鼠免疫功能的调节作用。方法:健康成年小鼠40只,随机分为对照组、环磷酰胺(CTX)免疫抑制组、黄蘑碱溶性多糖活性组分(Fb)正常小鼠给药组和Fb环磷酰胺伍用(M)给药组,采用NK细胞介导的细胞毒试验测定自然杀伤细胞(NK)的活性,淋巴细胞转化试验测定T淋巴细胞增殖功能,鼠脾T淋巴母细胞增殖分析法测定白细胞介素2(IL-2)的活性,观察Fb对各组小鼠免疫指标的影响。同时通过明胶酶谱分析检测脾脏基质金属蛋白酶(MMPs)的表达。结果:黄蘑多糖Fb能提高免疫抑制小鼠的NK细胞活性,T淋巴细胞转化率(LTT)和IL-2的活性(P<0.05),对正常小鼠LTT有显著促进作用(P<0.01)。正常给药组和伍用组主要免疫器官脾脏和胸腺重量高于免疫抑制组,且小鼠体重增长也明显高于免疫抑制组(P<0.01);免疫抑制组小鼠脾脏MMP-2活性下降(P<0.05),MMP-9亦呈降低趋势(P>0.05),Fb组和M组MMP-9活性明显升高(P<0.01)。结论:黄蘑多糖Fb能提高免疫抑制小鼠免疫系统的活性并有可能保护和促进其免疫系统的修复和增生,显著提高正常小鼠T淋巴细胞转化率,其他免疫指标亦显示轻度上调作用。Objective To study the regulatory effects of polysaccharide Fb from Huangmo （Fb） on immunological function in mice. Methods 40 healthy adult mice were randomly divided into control group （C）, CTX immunesuppressed group （CTX）, Fb treated group （Fb）, Fb combined with CTX treated group （M）. The influences of Fb in NK cell activity were determined by the method of NK mediated cytotoxicity test; the multiplicative capacity of lymphocyte was assayed by lymphocyte transformation rate （LTT） of T cell, and the mice spleen T lymphoblast multiplication analytical method was used to measure the IL-2 level. The expressions of MMPs in related immune system were measured by gelatin zymogram electrophoresis. Results Fb increased the NK cell activity, LTT and captivity of IL-2 in immune-suppressed mice （P〈0.05）, and also promoted the LTT in normal mice （P〈0.01）. The weights of spleen and thymus in Fb and M groups were increased, and the body weights of mice in Fb and M groups were increased remarkably than that in CTX group （P〈0. 01）. The activities of MMP-2 （P〈0.05） and MMP-9 （P 〈 0.05） indicated a decreased tendency in CTX group, the activity of MMP-9 were increased significantly in Fb group and M group （P〈0. 01）. Conclusion Fb has an activating effect on immune system and may protect and promote the proliferating and repairing of immune system in immune-suppressed mice. Fb can promote the LTT of T cell significantly and may up-regulate some other immune indexes slightly.

关 键 词：担子菌纲 多糖类/药理学 环磷酰胺 免疫 天然 明胶酶A 明胶酶B 

分 类 号：R392.1[医药卫生—免疫学] R-332[医药卫生—基础医学]