1,25(OH)_2D_3对大鼠单侧输尿管梗阻肾组织核因子κB表达的影响及意义  

Effect of 1, 25 (OH)_2D_3 on interstitial fibrosis in UUO rats

作　　者：姚刚[1] 许宏岳[1] 方奕[1] 武晓春[1] 

机构地区：[1]南京医科大学第二附属医院肾脏科,江苏南京210011

出　　处：《南京医科大学学报（自然科学版）》2005年第10期703-705,F0003,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金资助项目(NY1997023)

摘　　要：目的:探讨1,25(OH)2D3对肾组织纤维化的影响及作用机制。方法:雄性6周龄SD大鼠,建立单侧输尿管梗阻模型(UUO),设:①模型组(n=6),行左侧输尿管结扎术;②治疗组(n=6),1,25(OH)2D3溶于无水乙醇中腹腔注射,每100g体重3ng/d;③溶剂对照组(n=6),等容积无水乙醇腹腔注射;④假手术组(n=6)。第7天处死各组大鼠,采用光镜和RT-PCR观察肾组织纤维化和核因子(NF)-κB表达的变化。结果:治疗组大鼠肾间质纤维化程度较模型组和溶剂对照组明显减轻。UUO模型第7天时大鼠肾组织NF-κB表达明显增强,采用1,25(OH)2D3治疗后,肾组织NF-KB表达的异常增强能得到有效抑制。结论:1,25(OH)2D3具有阻抑慢性肾脏疾病进行性发展的潜能,而这一作用与其能有效降低肾组织NF-κB表达的异常增强有关。Objective ： To investigate the effects of 1,25 （OH）2D3 on renal interstitial fibrosis in rat. Methods： Male SD rats （7 weeks old） were divided into 4 groups at random： ①UUO group（6 rats）, ②the treated group （6 rats）： UUO ＋ 1,25（OH）2D3 per 100 g 3 ng/day by intraperitoneally （I.P）,③the control group（6 rats）： UUO ＋ ethanol by I.P, ④ sham operated group（6 rats）. Kidneys were harvested on day 7 after UUO. Fibrosis was assessed by measuring tissue hydroxyproline content. Tissue NF-κB expression was determined by RT-PCR. Results： UUO for 7 days significantly activated NF-κB,induced interstitial fibrosis in obstructed kidney of the control group and UUO group compared with sham operated group rats. Conversely daily administration of 1,25 （OH）2D3 significantly inhibited NF-κB expressionand decreased interstitial fibrosis in obstructed kidney of treated group. Conclusion： 1,25 （OH）2D3 might have had beneficial effects on interstitial fibrosis in SD rats with UUO, and this effect may be associated with 1,25 （OH）2D3 inhibits NF-κB expression.

关 键 词：肾脏 纤维化 1 25(OH)2D3 大鼠 

分 类 号：R322.61[医药卫生—人体解剖和组织胚胎学]