水通道蛋白2在人不同时期子宫内膜中的表达  

Expression of aquaporin 2,a water channel,in human endometrium during the menstrual cycle

作　　者：何荣环[1] 周彩云[1] 王波[1] 孙惠兰[1] 金帆[1] 黄荷凤[1] 

机构地区：[1]浙江大学医学院附属妇产科医院生殖内分泌科,杭州310006

出　　处：《中华医学杂志》2005年第34期2420-2424,共5页National Medical Journal of China

摘　　要：目的探讨水通道蛋白2(aquaporin 2,AQP2)在人不同时期子宫内膜中的表达特点及其意义。方法采用免疫组织化学技术、W estern印迹方法和逆转录-聚合酶链反应(RT-PCR)对87例(其中增生期23例,分泌早期30例,分泌中期34例)来自生育年龄妇女不同月经周期的子宫内膜行AQP2的检测。结果AQP2阳性着色主要分布于人子宫内膜腔上皮和腺上皮细胞的胞膜和胞质;W estern印迹证实了AQP2在人子宫内膜中的表达,半定量分析发现增生期内膜AQP2的相对表达量为1.03±0.10,显著低于分泌早期(1.33±0.14,P<0.05)和分泌中期(1.63±0.15,P<0.01);分泌早期和中期相比,差异有统计学意义(P<0.05)。结论AQP2在人子宫内膜中表达,且其表达量随月经周期而变化,表明其可能在性激素作用下的子宫内液体平衡过程中起一定作用。Objective To examine the expression of aquaporin 2 （AQP2） in human endometrium. Methods Specimens of human endometrium were collected from 87 women at different menstrual cycles, aged 30±3, 23 cases in the proliferative phase, 30 cases in the early secretory phase and 34 cases in the mid-secretory phase respectively. Immunohistochemistry and Western blotting were utilized to detect the localization and expression of AQP2 in the endometrium. Reverse transcription-polymerase chain reaction （RT-PCR） was employed to detect its messenger RNA. The PCR product was cloned and sequenced. Results Positive immunoreactivity of AQP2 was found in the epithelia cells and glandular epithelial cells of all specimens of human endometrium at different phases of the menstrual cycle, and all stromal cells were not stained. The reactive substance was primarily distributed in the membrane and cytoplasm, but not in the nuclei of all positive cells. In Western blotting showed dominant bands with relative molecular weight between 35 000 Da and 50 000Da, which was corresponded to the glycosylated form of AQP2 by the positive control from rat kidney. Semi-quantitative analysis showed that the relative expression of AQP2 in the midsecretory phase was 1.63±0. 15, significantly higher than those in the early secretory phase （ 1.33±0. 14, P 〈 0.05 ） and that in the proliferative phase （ 1.03±0. 10, P 〈 0.0! ）. Message RNA was found out in all cases by RT-PCR and the PCR product was confirmed in nearly exact （99%） consistency with the GenBank by sequencing. AQP2mRNA was expressed in all normal endometrium at different phases, and was weakly expressed in the endometrium at the proliferative phase. Sequencing showed that the AQP2 sequence was 99% homologous with that in the GenBank. Conclusion AQP2 expression in the human endometrium suggests that AQP2 may be involved in the regulation of uterine fluid homeostasis influenced by ovarian steroid hormones in the menstrual cycle.

关 键 词：女(雌)性 子宫内膜 水孔蛋白类 子宫内膜腔 水通道蛋白2 不同时期 Western印迹方法 逆转录-聚合酶链反应 免疫组织化学技术 分泌早期 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]