白玉凤尾蕨孢子繁殖技术的研究  

Studies on Spore Propagation of Pteris cretica 'Albo-lineata'

作　　者：徐艳[1] 石雷[1] 刘燕[2] 李东[1] 

机构地区：[1]中国科学院植物研究所,北京100093 [2]北京林业大学园林学院,北京100083

出　　处：《园艺学报》2005年第4期658-662,共5页Acta Horticulturae Sinica

基　　金：科技部国家科技基础条件平台工作项目(2004DKA30430);中国科学院农办项目(NK十五-C-13)

摘　　要：研究了白玉凤尾蕨(Pteris cretica'Albo-lineata')孢子的无菌培养和常规繁殖方法.结果表明:孢子萌发为书带蕨型,配子体发育为水蕨型.1/2 MS培养基中萌发率最高,可达82.3%;低于2%的蔗糖浓度较适于孢子的萌发;MS培养基中配子体成苗率最高,可达53.4%;试管苗移栽成活率可达80%以上.草炭土+河沙(体积比1∶ 1)是播种孢子的理想基质,配子体成苗率达89.2%;当幼孢子体苗的第1片子叶长至1～2 cm长时分苗,移栽到草炭土+蛭石+素砂+有机肥(体积比4∶ 2∶ 2∶ 1)的栽培基质上,移栽成活率最高,可达98.1%.幼孢子体苗长至10 cm左右时已经具有一定的观赏价值.The spore sterile culture and routine propagation of Pteris cretica ‘ Albo-lineata＇ were studied respectively. The results showed that the spore germination was of the Vittaria-type while the gametophyte development was of the Ceratopteris-type. The maximum germination rate was obtained in 1/2MS medium, which reached 82.3% ; the medium with sucrose concentration being less than 2% was appropriate for spore germination; the highest rate of gametophyte to sporophyte was achieved in MS medium, which reached 53.4% ; the transplant survival rate of sporophyte in vitro reached above 80%. The mixed soil （the turf and the sand blend in the volume ratio of 1 to 1 ） was the optimal growing medium for spore routine propagation, in which the rate of gametophyte to the sporophyte was up to 89. 2%. When the first cotyledon of the sporophyte seedling grew to 1-2 cm, the sporophyte seedlings should be replanted in the growing medium which was composed of turf, vermiculite, sand and organic young gametophyte seedlings of 10 cm height had fertilizers. The highest survival rate reached 98.1%. The already possessed ornamental values.

关 键 词：凤尾蕨 孢子 无菌培养 常规繁殖 

分 类 号：S682.35[农业科学—观赏园艺]