人巨细胞病毒UL141片段在临床低传代分离株中的多态性研究  

Polymorphism of human cytomegalovirus UL141 gene in clinical isolates of low passage

作　　者：齐莹[1] 阮强[1] 马艳萍[1] 何蓉[1] 吉耀华[1] 孙峥嵘[1] 黄郁晶[1] 刘庆[1] 陈淑荣[1] 王继东[1] 

机构地区：[1]中国医科大学附属第二医院病毒研究室,辽宁沈阳110004

出　　处：《中国医科大学学报》2005年第5期391-393,398,共4页Journal of China Medical University

基　　金：国家自然科学基因资助项目(30170986)

摘　　要：目的:研究人巨细胞病毒不同临床分离株UL141基因多态性,并分析这种多态性与人巨细胞病毒先天感染疾病之间的关系。方法:对荧光定量PCR方法检测HCMV-DNA阳性的临床低传代分离株进行UL141基因全序列PCR扩增,对扩增阳性的标本进行测序及分析。结果:19株临床低传代分离株在Toledo株UL141基因227位均缺失一个碱基T,因此产生两个新的ORF(UL141A和UL141B)。与Toledo株相应片段比较,UL141A预测蛋白质第75位氨基酸后序列和翻译后修饰位点产生大量变异。UL141B高度保守。结论:临床分离株的UL141片段产生两个新的ORF。Objective： To study the polymorphism of human cytomegalovirus （HCMV） UL141 in clinical isolates and find the relationship between the polymorphism and the outcome of congenital HCMV infection. Methods： PCR was performed to amplify the entire UL141 region of clinical isolates, which had been proven HCMV-DNA positive by real time PCR. The PCR products were sequenced and the data were analyzed, Results：There was a nueleotide deletion at the posi- tion 227 in clinical isohes, and 2 new UL141 open reading frames named UL141A and UL141B were found. In clinical isolates the predicted proteins of UL141A were hypervariable post the position 75 of amino acid sequence. The predictied.protein of UL141 B was highly conserved. Conclusion：There are two UL141 open reading frames in clinical isolates.

关 键 词：人巨细胞病毒 多态性 基因 

分 类 号：R373[医药卫生—病原生物学]