针对c-myc mRNA两个不同位点的c-myc反义寡脱氧核苷酸抑制类风湿滑膜细胞增殖的比较  

Inhibiting synoviocytes proliferation by two types of c-myc antisense oligodeoxynucleotides

作　　者：赵欣欣[1] 赵丽娟[1] 肖卫国[1] 

机构地区：[1]中国医科大学附属第一医院风湿免疫科,辽宁沈阳110001

出　　处：《中国医科大学学报》2005年第5期451-453,共3页Journal of China Medical University

基　　金：辽宁省科技厅科技攻关项目(00225001)

摘　　要：目的:研究针对c-myc mRNA不同位点的两种反义寡脱氧核苷酸序列(AS-ODN):myc-AUG AS-ODN,myc-CRD AS-ODN,对类风湿滑膜细胞增殖的抑制作用。方法:培养人类风湿滑膜B型细胞,合成反义寡脱氧核苷酸序列并进行硫代磷酸化修饰,以脂质体L ipofectin为载体,转染滑膜细胞,用MTT法检测细胞的增殖情况。结果:myc-AUG AS-ODN,myc-CRD AS-ODN均能抑制滑膜细胞增殖,使细胞数目减少的最大值分别为40.0%和41.4%。myc-AUG AS-ODN抑制滑膜细胞增殖的起效时间早于myc-CRD AS-ODN。在高浓度时寡脱氧核苷酸具有非序列特异性细胞毒作用。结论:两种AS-ODN序列均可用于抑制滑膜细胞增殖。Objective： To investigate the difference in inhibiting synoviocytes proliferation by 2 c-mye antisense oligodeoxynucleotides （AS-ODN） sequences, myc-AUG AS-ODN and myc-CRD AS-ODN. Methods： Human synoviocytes from patients with rheumatoid arthritis were cultured. The sequences were modified by phosphorothioates. Lipofectin was used as a acarrier. MTT assay was used to examine the inhibition of cell proliferation. Results： Both myc-AUG AS-ODN and myc-CRD AS-ODN inhibited synoviocytes proliferation. The maximal decrement of cell number were 40. 0% and 41.4%, respectively. The inhibiting time of myc-AUG AS-ODN was shorter than that of myc-CRD AS-ODN. ODN of high level had non-sequence-specific cytotoxicity. Conclusion： Both sequences of c-myc AS-ODN are useful in inhibiting synoviocytes proliferation.

关 键 词：类风湿 滑膜 反义寡脱氧核苷酸 

分 类 号：R593.2[医药卫生—内科学]