产朊圆酵母(Torula utilis Henn 2.281)尿酸酶快速提取法的研究  

Studies on Rapid Extraction of Uricase from Torula utilis Henneb 2.281

作　　者：余彩霞[1] 杨波[1] 辛俞[1] 谭晓晶[1] 许晓燕[1] 孟延发[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《四川大学学报（自然科学版）》2005年第5期1057-1060,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：The combination of ultrasonic and Triton X-100 and β-ME made it easy to release Uricase from Torula utilis Henneb 2.281 quickly when the pH of cell suspending mixture varied from 7 to 11.The results of our researches were obtained as follow : the cell suspending mixture obtained the highest enzyme activity(20.4U/mL),after being treated with by Triton X-100 and β-ME(5%,v/v) and ultrasonic.The enzyme activity obtained by this method was obviously higher than by single treating method,e.g.ultrasonic(7.6U/mL) or Triton X-100 and β-ME(15.2U/mL).The combination of ultrasonic and Triton X-100 and β-ME made it easy to release Uricase from Torula utilis Henneb 2.281 quickly when the pH of cell suspending mixture varied from 7 to 11. The results of our researches were obtained as follow ： the cell suspending mixture obtained the highest enzyme activity （20.4U/mL） ,after being treated with by Triton X-100 and β-ME （ 5% , v/v ） and ultrasonic. The enzyme activity obtained by this method was obviously higher than by single treating method, e.g. ultrasonic （7.6U/mL） or Triton X-100 and β-ME （15.2U/mL）.

关 键 词：产朊圆酵母 尿酸酶 破碎技术 细胞壁 提取方法 

分 类 号：Q55[生物学—生物化学]