甘蓝型油菜(Brassica napus)中一个未知功能蛋白基因的cDNA克隆  

The cDNA Cloning of a Gene Encoding an Unknown Protein from Brassica napus

作　　者：邱峰[1] 王茂林[1] 刘阳[1] 周云涛[1] 赵云[1] 张帆[2] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川省种子管理站,成都610041

出　　处：《四川大学学报（自然科学版）》2005年第5期1066-1068,共3页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30170500)

摘　　要：According to the sequence of a 352bp fragment-M17 from the suppression subtractive hybridization library of the mutant line Cr3529 of Brassica napus,primers were designed and a 1000bp upstream fragment was cloned by 5’-RACE,which was joined with the original piece and composed a 1343bp sequence.According to the 3’ and 5’ ends sequences,a pair of primers were designed and the encoding region sequence of this unknown protein was amplified and cloned.Sequence analyzing showed that it had 80.1% identity with an unknown-protein-encoding gene from Arabidopsis thaliana.Swiss-model was used to predict the structure of the induced protein,and a potential transmembrane helix from site 46 Leu to 66 Phe was identified.GLOBE prediction of globularity showed that this protein possessed a compact globular domain.According to the sequence of a 352bp fragment-M17 from the suppression subtractive hybridization library of the mutant line Cr3529 of Brassica napus,primers were designed and a 1000bp upstream fragment was cloned by 5′-RACE,which was joined with the original piece and composed a 1343bp sequence.According to the 3′ and 5′ ends sequences, a pair of primers were designed and the encoding region sequence of this unknown protein was amplified and coloned.Sequence analyzing showed that it had 80.1% identity with an unknown-protein-encoding gene from Arabidopsis thaliana.Swrss-model was used to predict the structure of the induced protein,and a potential transmembrane helix from site 46 Leu to 66 Phe was identified.GLOBE prediction of globularity showed that this protein possessed a compact globular domain.

关 键 词：甘蓝型油菜 蛋白基因 CDNA 克隆技术 分子生物学 

分 类 号：Q949.4[生物学—植物学]