荧光法测定微藻中色氨酸的含量  

Fluorescence measurement of tryptophan content in microalgae

作　　者：吴芳[1] 郭卫东[1] 张润[1] 夏恩琴[1] 周宽波[1] 

机构地区：[1]厦门大学海洋系亚热带海洋研究所,福建厦门361005

出　　处：《海洋科学》2005年第10期1-4,共4页Marine Sciences

基　　金：国家自然科学基金资助项目(40106007)

摘　　要：建立了微藻中色氨酸的荧光分析方法.以NaOH为水解液水解微藻,在激发波长225nm,发射波长350 nm处测定色氨酸的荧光强度.方法线性范围0～0.07 mg/L,检测限为0.003 mg/L,回收率90%～100.1%.此法快速、简便、灵敏度高.This paper set up a procedure of fluorescence method to measure tryptophan in microalgae. NaOH containing 0.5 % soluble starch was used as hydrolytic solution. The fluorescence intensity of tryptophan was determined with excitation wavelength 225 nm and emission wavelength 350 nm. The linear range of calibration graph was 0-0.07 mg/L. The detection limit was 0. 003 mg/L. The recovery rate of sample was 90%-100.1%.

关 键 词：色氨酸 微藻 荧光法 

分 类 号：Q503[生物学—生物化学]