高效液相色谱法测定益泼丰的含量  

AN ASSAY OF IPRIFLAVONE BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：米慕真[1] 高文兰[1] 

机构地区：[1]沈阳药科大学基础部

出　　处：《沈阳药科大学学报》1996年第2期117-120,共4页Journal of Shenyang Pharmaceutical University

摘　　要：本文报道了ＨＰＬＣ内标法加校正因子测定法测定益泼丰原料药中益泼丰的含量。选ＯＤＳ色谱柱，以醋酸曲安缩松为内标物，甲醇：水（７２：２８）为流动相。益泼丰对照品与内标物的量之比对其色谱峰面积比作图，线性关系良好，相关系数ｒ＝０．９９９８，平均回收率为９９．８％。相对标准差ＲＳＤ为０．４％（ｎ＝１５）．平均校正因子ｆ＝０．２７６４，ＲＳＤ＝０６％（ｎ＝５）．方法简便，快速，准确。This report describes an HPLC method of internal standardization with correction factor for determining quantitatively the contents of Iprifiavone in its raw material.An ODS column and a mobile phase of methanol and water(72:28) were used and Triamcinoloni acetas was selected as the internal standard.The linear relationship between the quantitative ratio of the standard of Iprifiavone to the internal standard and the ratio of their peek area is quite good. The correlation coefficient is 0.9998,the average recovery is 99.8%,and RSD is 0.4%(n=15).This method is rapid and easy,the accuracy is quite good.It is a practical method for the assay of Iprifiavone raw material.

关 键 词：益泼丰 醋酸曲安缩松 高效液相色谱 

分 类 号：R97[医药卫生—药品] R927.2[医药卫生—药学]