自体移植皮片中黑皮素-1受体mRNA的表达及意义  

Expression of melanocortin-1 receptor mRNA and its significance in human skin autografts

作　　者：薛春雨[1] 邢新[1] 李蠡[1] 李军辉[1] 蒋平[2] 

机构地区：[1]第二军医大学长海医院整形外科,上海市200433 [2]第二军医大学生物化学与分子生物学教研室

出　　处：《中国美容医学》2005年第5期620-622,共3页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助项目(30371473)

摘　　要：目的:探讨黑皮素-1受体在自体移植皮片过度色素沉着中的作用。方法:利用RT-PCR、Masson-Fontanastain方法检测人体自体中厚移植皮片与原供区及受区周围自体正常对照皮肤中黑皮素-1受体mRNA的表达及表皮中黑色素的含量,并行统计学分析。结果:黑皮素-1受体在自体中厚移植皮片中的mRNA表达明显高于自身正常对照皮肤,其表达差异有显著性意义(P<0.01);自体中厚移植皮片表皮中黑色素含量较自身正常对照皮肤表皮中的黑色素含量亦明显增多,其含量的差异亦有显著性意义(P<0.01);且表皮中黑色素的含量与黑皮素-1受体的mRNA表达量呈正相关。结论:黑皮素-1受体在自体移植皮片过度色素沉着中起重要调控作用。Objective To elucide the role of MC-1R in hyperpigmented process of skin autografts. Methods RTPCR technique was performed to detect the expression of MC-1R mRNA in medium thickness skin autografts and normal skin respectively;Masson-Fontana stain technique was performed to detect the contents of melanin in medium thickness skin autografts and normal skin epidermis respectively. Statistics was used to analyze the data. Results The expression of MC-1R mRNA in medium thickness skin autografts was much stronger than that of control normal skin.The contents of melanin in medium thickness skin autografts epidermis was obvious increase than that of control normal skin.The expression of MC-IR mRNA was positive correlated with the contents of melanin in epidermis, Conclusion Overexpression of MC-IR in medium thickness skin autografts may play a important role in hyperpigmented process of skin auto qrafts.

关 键 词：黑皮素-1受体 自体移植皮片 过度色素沉着 

分 类 号：R392.4[医药卫生—免疫学]