超抗原SEA增强小鼠对HBV DNA疫苗的免疫反应  

Enhancement of Immune Responses to Hepatitis B DNA Vaccine by Superantigen SEA in Mice

作　　者：靳彦文[1] 李平[1] 胥全彬[1] 刘萱[1] 黄维[1] 王云龙 曹诚[1] 马清钧[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850 [2]河南生物工程技术研究中心,郑州450001

出　　处：《生物工程学报》2005年第5期681-685,共5页Chinese Journal of Biotechnology

基　　金：国家高技术研究与发展项目基金资助(No.2004AA215230)。~~

摘　　要：观察超抗原SEA(D227A)的真核表达载体(pmSEA),对HBVDNA疫苗诱导Balbc小鼠(H2d)免疫应答的调节作用。肌内注射空载体pcDNA3、HBVDNA疫苗加pmSEA佐剂(pHBVS2S+pmSEA)或不加佐剂(pHBVS2S);ELISA法测定血清抗HBs;ELISPOT检测分泌IFNγ的脾淋巴细胞;4h51Cr释放法检测小鼠脾细胞CTL活性。HBVDNA佐剂组免疫小鼠抗HBsAg抗体滴度明显高于不加佐剂组,其IgG1IgG2a的比例不同于多肽免疫组,二者分别为0.282与10。HBVDNA佐剂组均能增强IgG1和IgG2a的产生,是不加佐剂组的1.36、1.73倍。佐剂组小鼠脾淋巴细胞IFNγ的分泌量是不加佐剂组2～3倍。CTL细胞杀伤活性(E:T=100)佐剂组与不加佐剂组分别为:69.77%±7.5%、42.81%±7.7%,差异显著(P<0.05)。HBVDNA疫苗具有较强的免疫原性,能够诱导机体产生特异性的抗体及CTL反应;pmSEA佐剂能够提高小鼠对DNA疫苗的免疫应答,有望成为DNA疫苗的免疫佐剂。To investigate the adjuvant effect of plasmid DNA encoding superantigen SEA （D227A） （pmSEA） on immune responses induced by HBV DNA vaccine containing HBV preS2 and S antigen in BABL/c （H-2^d）. BALB/c mice were immunized intramuscular injection with HBV DNA vaccine （pHBVS2S） mixed with or without pmSEA plasmid. Antibodies againat HBV PreS2 and S antigen in the sera were accessed by Anti-HBs ELISA, and the HBsAg specific cytotoxic T lymphocytes （CTLs） activity was determined by 51 Chromium Release Assay. The HBs peptide-specific IFN-γ secreting T cells were detected by ELISPOT.Anti-HBs antibody titers and CTLs activity in mice immunized with pmSEA ＋ pHBVS2S group were significant higher （ P 〈0. 05） than pttBVS2S DNA vaccine group. The ratio of IgG1/IgG2a （0.282） was apparently different from the group immunized with peptide （10）. Mice immunized with HBV DNA vaccine plus adjuvant produce higher titer of IgG1 and IgG2a antibodies against HBV S antigen 1.36 and 1.73 time higher than that without adjuvant respectively. HBs peptide - specific IFN-7 secreting T cells increased 2 - 3 times by the pmSEA adjuvant, compared to DNA vaccine group. HBV DNAvaccine （pHBVS2S） induces humoral and cellular immuno-responses in BALB/c mice, and the responses could be significantly boasted by the plasmid encoding mSEA. Therefore the pmSEA was a potential adjuvant for DNA vaccines.

关 键 词：DNA疫苗 乙型肝炎表面抗原 SEA 小鼠 佐剂 

分 类 号：R392.11[医药卫生—免疫学]