西藏地区土壤放线菌种群多样性及拮抗活性研究  

Study on population diversity and antimicrobial activity ofactinomycete from acidic soil in Xizang area

作　　者：罗红丽 [1] 黄英 [2] 王黎明 [2] 刘志恒 [2] 谢建平 [3] 胡昌华 [3] 

机构地区：[1]西南师范大学生命科学学院,现代生物医药研究所,重庆,400715,中国科学院微生物研究所,微生物资源前期开发国家重点实验室,北京,100080 [2]中国科学院微生物研究所,微生物资源前期开发国家重点实验室,北京,100080 [3]西南师范大学生命科学学院,现代生物医药研究所,重庆,400715

出　　处：《微生物学报》2005年第5期724-727,共4页Acta Microbiologica Sinica

基　　金：国家"863计划"(2004AA227100)~~

摘　　要：从西藏不同海拔、不同气候条件的5个地区采集的10份土样中,使用选择分离培养基、ISP2(Yeast extract-malt extract agar)和高氏一号培养基,通过分散差速离心法(Dispersion and differential centrifugation,DDC)分离得到放线菌156株。根据形态和培养特征将其归入32个类群,并从中选出65个代表菌株进行全细胞壁氨基酸组分分析,结果表明:9株菌为非链霉菌胞壁类型,其余为链霉菌胞壁类型。16SrDNA扩增片段长度多态性(ARDRA)分析得到不同带型,对其序列分析结果表明:分离菌株分属链霉菌属(Streptomyces)和5个稀有放线菌属。同时测试了代表菌株抗耐药细菌和真菌的活性,其中38.5%的菌株具有拮抗活性。156 actinomycetes were isolated from 10 soil samples collected in Tibet by the method of DDC（dispersion and differential centrifugation） . The isolates were assigned to 32 different groups on the basis of their morphological characters and colors of substrate mycelium, aerial hyphae and pigment. The result of cell-wall DAP on 65 representative strains selected from these groups was that 9 strains were different from Streptomyces,The analysis of ARDRA and 16S rDNA sequeces showed that the isolates belonged to the genus Streptomyces and five different rare actinomycete genera respectively. Antimierobial activity of the representative strains was also tested, with the result that 38.5 % isolates were positive.

关 键 词：西藏地区 放线菌 ARDRA 种群多样性 拮抗活性 

分 类 号：S154.3[农业科学—土壤学]