重组质粒在减毒沙门氏菌中的稳定性及其对细菌侵袭力的影响  

Stability of recombinant plasmids in attenuated Salmonella typhimurium and their effects on bacterial invasiveness and multiplication

作　　者：陈雪燕[1] JOHN M.Dikki 江玲丽[1] 帅江冰[1] 方维焕[1] 

机构地区：[1]浙江大学动物预防医学研究所浙江省动物预防医学重点实验室,杭州310029

出　　处：《微生物学报》2005年第5期744-747,共4页Acta Microbiologica Sinica

基　　金：浙江省自然科学基金重大项目(ZA0105)~~

摘　　要：将含有外源基因的重组真核表达质粒pcDNA3-F和pCI-F转化减毒鼠伤寒门氏菌,探讨质粒类型和插入片段对重组质粒在细菌内的稳定性和细菌侵袭力的影响。结果表明,外源质粒可降低减毒沙门氏菌在体外的增殖能力和侵袭力,也影响细菌在鸡体内的存活力;就质粒类型而言,pCI的影响大于pcDNA3,而以携带外源基因的重组质粒影响较为显著;外源基因插入也影响质粒在宿主菌内的稳定性。提示利用减毒鼠伤寒沙门氏菌为载体传递DNA疫苗研究时,要考虑质粒类型与其在宿主菌内稳定性的关系、携带外源基因重组质粒对载体菌侵袭力和存活力的影响等问题。Effects of plasmid type and insertion sequence on in vitro and in vivo multiplication and invasiveness of attenuated Salmonella typhimurium were examined following transformation of the bacteria with eukaryotic expression plasmids pcDNA3 and pCI with or without heterologous gene （Newcastle disease virus F gene） . Exogenous plasmids had negative impacts on the replication or invasiveness of the attenuated S. typhimurium in LB broth and/or HeLa cell monolayers as well as on its survival in live chicks. The plasmid pCI had more significant effects than pcDNA3. Introduction of heterologous gene into the plasmids not only exhibited additional negative influence on the host strain but also on their own stability therein. All these results suggest that full consideration should be given to the types of plasmids and their stability within the host strain as well as to their effects on replication and invasiveness of attenuated bacteria as the DNA vaccine delivery vector for improved immune protection.

关 键 词：减毒沙门氏菌 侵袭力 重组真核表达质粒 稳定性 

分 类 号：Q789[生物学—分子生物学]