咔唑分光光度法测定硫酸软骨素  

Spectrophotometric Determination of Chondroitin Sulfate Using Carbazole

作　　者：于兹东[1] 杨桂明[1] 高华[1] 刘坤[1] 

机构地区：[1]青岛大学医学院化学教研室,山东青岛266021

出　　处：《青岛大学学报（自然科学版）》2005年第3期41-44,共4页Journal of Qingdao University(Natural Science Edition)

基　　金：山东省教育厅资助项目(J00C59)

摘　　要：硫酸软骨素在硫酸溶液中加热水解生成等摩尔数的葡萄糖醛酸,与咔唑溶液发生缩合反应生成紫红色化合物,经分光光度法测定其最大吸收波长为520 nm,线性范围为0～0.30 g/L(r=0.9998),回收率为99.10% ,相对标准偏差为0.91% .To determine mass concentration of chondroitin sulfate , a new spectrophotometry is presented using carbazole. Chondroitin sulfate was hydrolyzed to produce a equal mole glucuronic acid product in hot sulfuric acid. The glucuronic acid was colored by carbazole. The colored solution has a maximum absorption peak at 520nm,its linear concentration is 0-0.30 g/L. The produt average recovery is 99. 10%, the relative standard deviation is 0.91%.

关 键 词：硫酸软骨素 咔唑 分光光度法 

分 类 号：Q538[生物学—生物化学]