长期固定使用一试剂“船”对均相法测定血清高密度脂蛋白胆固醇影响的研究  

Study on the Influence of Long-term a Reagent "Ship" of Stationary Use for the Homogenous Assay Measurement Serum High Density Lipoprotein Cholesterol

作　　者：赵庆昌[1] 张德[1] 王金兰[1] 蔡庆岭[1] 齐子芳[1] 

机构地区：[1]山东聊城市第二人民医院,山东临清252601

出　　处：《现代检验医学杂志》2005年第5期27-29,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨长期固定使用一试剂“船”对均相法测定血清高密度脂蛋白胆固醇(HDL-C)时所出现问题的原因,并寻找解决问题的办法。方法用出现问题的试剂“船”,装入一套新开启的HDL-C测定试剂,用出现问题的试剂和新开启试剂交叉组成两套试剂,模拟日常实际工作,分别用这些试剂测定质控血清和病人血清的HDL-C值。结果将测定结果经配对t检验,发现装入出现问题的试剂“船”中新开启的HDL-C试剂,前两天测定结果无明显变化(P>0.05);第三天开始发生明显变化,第四天变化最显著(P<0.01)。出现问题的试剂中试剂2的变化要比试剂1大(P<0.01)。结论要定期更换试剂“船”。Objective To study the cause of the long-term a reagent “ship” used fixs on for homogeneous assay measurement serum high density lipoprotein cholesterol （HDL-C）to the problem appeared and look for the problem-solving way. Methods The reagent “ship” that arised problem were used,with packing into a set of new-opened HDL-C reagent. The HDL-C reagent that arised problem and newly openning HDL-C ragent cross to constitute two sets new reagents. Imitating to daily actual work,with these reagents respectively measurement the quality control serum and the patient serum HDL- C value. Results Measurement result by t test,discovering to pack new-opened HDL-C reagents into the reagent “ship” of appeared problem,the measurement results were not significantly changed in the first two days （P〉0. 05） ;it was significantly changed in the third day,the most changed in the fourth day（P〈0. 01）. The change of reagent 2（R2） in reagent that arised problem was more serious than reagent 1（R1）（P（0. 01）. Conclusion Periodically replace the reagent “ship” is important.

关 键 词：均相法 HDL-C试剂盒 试剂“船” 

分 类 号：R446[医药卫生—诊断学]