慢性染铅对小鼠海马Ca^(2+)-钙调蛋白依赖性蛋白激酶Ⅱ表达的影响  

Effect of lead exposure on expression of Ca~ 2+-calmodulin dependent protein kinaseⅡ in mice

作　　者：文涛[1] 孙黎光[1] 宗志宏[1] 邢伟[1] 刘素媛[1] 

机构地区：[1]中国医科大学生物化学与分子生物学教研室,辽宁沈阳110001

出　　处：《中国药理学与毒理学杂志》2005年第5期393-395,共3页Chinese Journal of Pharmacology and Toxicology

基　　金：国家自然科学基金资助项目(39970651)~~

摘　　要：目的通过检测海马Ca2+钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)基因表达来探讨慢性铅中毒影响学习记忆的分子机制。方法小鼠交配后,通过饮水饲以2.4,4.8和9.6mmol·L-1醋酸铅。小鼠子代自胚胎期始即暴露于醋酸铅。幼鼠出生后,先通过哺乳接触铅,断乳后则自行饮用与母鼠饮用浓度相同的含铅水。6周后用逆转录聚合酶链反应法观察各组小鼠海马CaMKⅡmRNA的表达。结果3个染铅组小鼠CaMKⅡmRNA水平均明显降低,并具有浓度效应关系。结论铅致CaMKⅡ基因表达水平下降。AIM To explore the effect of chronic lead contaminant on learning and memory by detecting Ca^2＋ -calmodulin dependent protein kinase Ⅱ （CaMK Ⅱ） mRNA in hippocampus of mice. METHODS Newborn mice were given acetic lead in drinking water for 6 weeks. RT-PCR was used to observe the expression of CaMK Ⅱ mRNA in hippocampus. RESULTS Expression of CaMK Ⅱ in hippocampus was inhibited by acetic lead exposure in dose-dependent manner. The expression in 2.4, 4.8 and 9.6 mmol·L^-1 aceticlead groups was 83.6%, 61.5% and 36.7%, respectively, compared with control group （100%）. CONCLUSION Lead exposure can induce decrement of CaMK Ⅱ gene expression in hippocampus.

关 键 词：铅 Ca^2+-钙调蛋白依赖性蛋白激酶 海马 

分 类 号：R595.2[医药卫生—内科学]