桃幼胚及幼胚子叶再生的研究  

Plantlets Regeneration from Immature Embryos and Cotyledons of Peach

作　　者：吴延军[1] 张上隆[1] 谢鸣[2] 陈俊伟[2] 秦永华[1] 秦巧平[1] 

机构地区：[1]浙江大学园艺系,杭州310029 [2]浙江省农业科学院园艺所,杭州310021

出　　处：《林业科学》2005年第5期45-50,i0002,共7页Scientia Silvae Sinicae

基　　金：宁波市科委项目"桃转基因研究"(2000农业项目资助);浙江省农业科学院人才引进项目资助。

摘　　要：以玉露桃和湖景蜜露桃幼胚及幼胚子叶为试材,研究基本培养基类型、激素、损伤方式等因素对再生的影响。结果表明:培养基是影响幼胚诱导愈伤组织的最重要因素,MS适合供试材料诱导愈伤组织;激素诱导愈伤组织因品种而异,NAA1.0mg·L-1对45d、0.5mg·L-1对55d玉露,BA0.5mg·L-1对55d湖景蜜露效果较好,在试验浓度范围内2,4-D对供试幼胚均无明显效果;幼胚的发育状态是影响诱导愈伤组织的另一因素,玉露桃45d愈伤组织诱导率最高,达96.6%,而55d诱导率为81.6%。愈伤组织可在MS+0.05mg·L-1NAA+1.0mg·L-1BA培养基上分化成芽,再生芽在1/2MS+1.0mg·L-1IBA培养基上生根。幼胚子叶不定芽再生培养基的激素配比为NAA0.50mg·L-1+BA10.0mg·L-1、NAA0.05mg·L-1+TDZ3.0mg·L-1;带胚芽子叶纵向刻伤再生率高;不定梢(芽)在1/2MS+2%蔗糖+7.5g·L-1琼脂+IBA1.0mg·L-1+Ad20mg·L-1的培养基上能生根。The main composition of culture media such as the basic medium and hormones were tested for their effects on Peach （Prunus persica ） callus frequency from immature embryos （which picked after full flower 4.5 days and 55 days respectively） under （26± 1） ℃ without light in an orthogonal experiment. And a method for plant regeneration from immature cotyledons （55 d） has been developed by cultured on MS media supplemented with 2% sucrose and 0.75% agar and hormone in various combinations. The results showed that： The basal medium was the most important factor for inducing callus from embryos. MS was adapted to ‘Yulu＇ （45 d and 55 d） and ‘Hujingmilu＇ （55 d） . NAA 1.0 mg·L^-1 and 0.5 mg·L^-1 were better for ‘Yulu＇ （4.5 d and 55 d, respectively）, BA 0.5 mg·L^- 1 was better for ‘Hujingmilu＇ （55 d）, but 2,4-D hadn＇t any important effect on callus inducing from the examined material. Days after fecundation was another factor effect on callus induction. The highest callus frequency from 4.5 d immature embryos was 96.6% and it was 81.6% from 55 d embryos. Shoots regeneration occurred when callus was transferred to a medium in which the NAA concentration was 0.0.5 mg·L^-1 and BA was 1.0 mg·L^-1 . Shoot regenerated roots in the medium of 1/2 MS ＋ 1.0 mg·L^-1 IBA. 2 types of hormone combinations （NAA 0.50 mg·L^-1 ＋ BA 10.0 mg·L^-1 , NAA 0.0.5 mg·L^-1 ＋ TDZ 3.0 mg·L^-1 ） were favorable to shoots differentiation for the immature cotyledons. The regeneration was changed when cotyledons were wounded horizontally by a razor blade. The best results were obtained when cotyledons with embryonic axis and wounded in the surface. Adventitious shoot could regenerate on the 1/2MS medium with IBA 1.0 mg·L^-1 andAd20 mg·L^-l.

关 键 词：桃 幼胚 子叶 愈伤组织 离体再生 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]