淡紫拟青霉菌IPC菌株原生质体形成和再生条件  

Formation and regeneration of the protoplasts of Paecilomyces lilacinus strain IPC

作　　者：张延新[1] 刘开启[2] 夏振远[3] 李天飞 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018 [2]仲恺农业技术学院资源环境系,广东广州510225 [3]云南省烟草科学研究所,云南玉溪653100 [4]云南省玉溪红塔集团,云南玉溪653100

出　　处：《植物保护》2005年第5期42-45,共4页Plant Protection

基　　金：广东省自然基金项目(021923);云南省"十五"攻关项目(2001NG08);国家烟草专卖局科技项目(110200101010)

摘　　要：研究了菌龄、渗透压稳定剂、酶的种类及浓度、酶解时间和再生培养基等因素对淡紫拟青霉菌IPC菌株原生质体形成和再生的影响,结果表明,IPC菌株原生质体形成的最适宜条件为:菌龄20h,用5 mg/mL蜗牛酶,0.8 mol/L NaCl的渗透压稳定剂,酶解2～3 h;原生质体涂布于含0.6 mol/L NaCl、0.5%琼脂的再生培养基上,再生率最高.The effects of such factors as mycelial age, osmotic stabilizers, enzymes and their concentration, lysis time and regeneration media on the formation and regeneration of the protoplasts of Paecilomyces lilacinus were studied. The optimum conditions are as follows： mycelia of 20h, 5mg/mL snailase, 0.8mol/L NaCl as osmotic stabilizer and lysis time of 2 -3h. The regeneration percentage was the highest when the protoplasm were plastered on 0. 596 agar media with 0.6 mol/L NaCl.

关 键 词：生物工程 淡紫拟青霉菌 原生质体再生 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]